| Literature DB >> 15593126 |
Winfried Barchet1, Anne Krug, Marina Cella, Celeste Newby, Jens A A Fischer, Andrzej Dzionek, Andrew Pekosz, Marco Colonna.
Abstract
Natural interferon-producing cells (IPC) secrete type I IFN (IFN-alpha and -beta) in response to influenza virus. This process is independent of viral replication and is mediated by Toll-like receptor 7 (TLR7), which recognizes single-stranded RNA (ssRNA). DC also express TLR7 but its function in DC response to influenza virus is unknown. To address this, we compared the DC and IPC responses to influenza virus and ssRNA oligoribonucleotides (ORN) that activate TLR7. When stimulated by ORN in vitro and in vivo, DC matured and produced inflammatory cytokines but not IFN-alpha. DC did secrete IFN-alpha in response to influenza virus. However, this response was independent of TLR7 signaling and required viral replication but not dsRNA-activated protein kinase (PKR). We conclude that DC and IPC are hard-wired to secrete IFN-alpha via different pathways, reflecting their complementary but distinct roles in anti-viral immunity.Entities:
Mesh：
Substances：
Year: 2005 PMID： 15593126 DOI： 10.1002/eji.200425583
Source DB: PubMed Journal: Eur J Immunol ISSN： 0014-2980 Impact factor: 5.532