Literature DB >> 15583329

Evaluation of decontamination methods and growth media for primary isolation of Mycobacterium ulcerans from surgical specimens.

Dorothy Yeboah-Manu1, Thomas Bodmer, Ernestina Mensah-Quainoo, Samuel Owusu, David Ofori-Adjei, Gerd Pluschke.   

Abstract

We evaluated four decontamination methods and one nondecontamination procedure in combination with four egg-based media for the primary isolation of Mycobacterium ulcerans from tissue specimens. With mycobacterial recovery and contamination rates of 75.6 and 2.4%, respectively, the combination of the oxalic acid decontamination method with Lowenstein-Jensen medium supplemented with glycerol yielded the best results.

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Year:  2004        PMID： 15583329      PMCID： PMC535292          DOI： 10.1128/JCM.42.12.5875-5876.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Development of a PCR assay for rapid diagnosis of Mycobacterium ulcerans infection.

Authors:  B C Ross; L Marino; F Oppedisano; R Edwards; R M Robins-Browne; P D Johnson
Journal:  J Clin Microbiol       Date:  1997-07       Impact factor: 5.948

2.  Effects of decontamination methods and culture conditions on viability of Mycobacterium ulcerans in the BACTEC system.

Authors:  J C Palomino; F Portaels
Journal:  J Clin Microbiol       Date:  1998-02       Impact factor: 5.948

Review 3.  Mycobacterium bohemicum cervical lymphadenitis.

Authors:  Astrid Palca; Christoph Aebi; Rosemarie Weimann; Thomas Bodmer
Journal:  Pediatr Infect Dis J       Date:  2002-10       Impact factor: 2.129

4.  Direct detection and identification of Mycobacterium ulcerans in clinical specimens by PCR and oligonucleotide-specific capture plate hybridization.

Authors:  F Portaels; J Agular; K Fissette; P A Fonteyne; H De Beenhouwer; P de Rijk; A Guédénon; R Lemans; C Steunou; C Zinsou; J M Dumonceau; W M Meyers
Journal:  J Clin Microbiol       Date:  1997-05       Impact factor: 5.948

5.  Buruli ulcer disease in Cameroon rediscovered.

Authors:  Jürgen Noeske; Christopher Kuaban; Simona Rondini; Philippe Sorlin; Laura Ciaffi; Josephine Mbuagbaw; Françoise Portaels; Gerd Pluschke
Journal:  Am J Trop Med Hyg       Date:  2004-05       Impact factor: 2.345
  5 in total
  27 in total

1.  Optimized method for preparation of DNA from pathogenic and environmental mycobacteria.

Authors:  Michael Käser; Marie-Thérèse Ruf; Julia Hauser; Laurent Marsollier; Gerd Pluschke
Journal:  Appl Environ Microbiol       Date:  2008-12-01       Impact factor: 4.792

2.  Fine-needle aspiration, an efficient sampling technique for bacteriological diagnosis of nonulcerative Buruli ulcer.

Authors:  Miriam Eddyani; Alexandra G Fraga; Fernando Schmitt; Cécile Uwizeye; Krista Fissette; Christian Johnson; Julia Aguiar; Ghislain Sopoh; Yves Barogui; Wayne M Meyers; Jorge Pedrosa; Françoise Portaels
Journal:  J Clin Microbiol       Date:  2009-04-22       Impact factor: 5.948

3.  Isolation of Mycobacterium ulcerans from swab and fine-needle-aspiration specimens.

Authors:  Dorothy Yeboah-Manu; Emelia Danso; Kobina Ampah; Adwoa Asante-Poku; Zuliehatu Nakobu; Gerd Pluschke
Journal:  J Clin Microbiol       Date:  2011-03-16       Impact factor: 5.948

4.  Isolation and characterization of nontuberculous mycobacteria from patients with pulmonary tuberculosis in Ghana.

Authors:  Isaac Darko Otchere; Adwoa Asante-Poku; Stephen Osei-Wusu; Samuel Yaw Aboagye; Dorothy Yeboah-Manu
Journal:  Int J Mycobacteriol       Date:  2017 Jan-Mar

5.  Genetic diversity in Mycobacterium ulcerans isolates from Ghana revealed by a newly identified locus containing a variable number of tandem repeats.

Authors:  Markus Hilty; Dorothy Yeboah-Manu; Daniel Boakye; Ernestina Mensah-Quainoo; Simona Rondini; Esther Schelling; David Ofori-Adjei; Françoise Portaels; Jakob Zinsstag; Gerd Pluschke
Journal:  J Bacteriol       Date:  2006-02       Impact factor: 3.490

6.  Primary culture of Mycobacterium ulcerans from human tissue specimens after storage in semisolid transport medium.

Authors:  Miriam Eddyani; Martine Debacker; Anandi Martin; Julia Aguiar; Christian R Johnson; Cécile Uwizeye; Krista Fissette; Françoise Portaels
Journal:  J Clin Microbiol       Date:  2007-11-07       Impact factor: 5.948

7.  Isolation of Nontuberculous Mycobacteria from the Environment of Ghanian Communities Where Buruli Ulcer Is Endemic.

Authors:  Samuel Yaw Aboagye; Emelia Danso; Kobina Assan Ampah; Zuliehatu Nakobu; Prince Asare; Isaac Darko Otchere; Katharina Röltgen; Dzidzo Yirenya-Tawiah; Dorothy Yeboah-Manu
Journal:  Appl Environ Microbiol       Date:  2016-06-30       Impact factor: 4.792

8.  Screening of antifungal azole drugs and agrochemicals with an adapted alamarBlue-based assay demonstrates antibacterial activity of croconazole against Mycobacterium ulcerans.

Authors:  Nicole Scherr; Katharina Röltgen; Matthias Witschel; Gerd Pluschke
Journal:  Antimicrob Agents Chemother       Date:  2012-09-24       Impact factor: 5.191

9.  Lack of insertional-deletional polymorphism in a collection of Mycobacterium ulcerans isolates from Ghanaian Buruli ulcer patients.

Authors:  Michael Käser; Oliver Gutmann; Julia Hauser; Tim Stinear; Stewart Cole; Dorothy Yeboah-Manu; Gregor Dernick; Ulrich Certa; Gerd Pluschke
Journal:  J Clin Microbiol       Date:  2009-09-02       Impact factor: 5.948

10.  Genomic diversity and evolution of Mycobacterium ulcerans revealed by next-generation sequencing.

Authors:  Weihong Qi; Michael Käser; Katharina Röltgen; Dorothy Yeboah-Manu; Gerd Pluschke
Journal:  PLoS Pathog       Date:  2009-09-11       Impact factor: 6.823
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