| Literature DB >> 15581618 |
Tao Chen1, Yan-Ling Zhang, Yan Jiang, Shu-Zhen Liu, Heide Schatten, Da-Yuan Chen, Qing-Yuan Sun.
Abstract
To study the DNA methylation events in normal and cloned rabbit embryos, we investigated the methylation status of a satellite sequence and the promoter region of a single-copy gene using bisulfite-sequencing technology. During normal rabbit embryo development, both sequences maintained hypermethylation status until the 8- to 16-cell stage when progressive demethylation took place. In cloned embryos, the single-copy gene promoter sequence was rapidly demethylated and precociously de novo methylated, while the satellite sequence maintained the donor-type methylation status in all examined stages. Our results indicate that unique sequences as well as satellite sequences may have aberrant methylation patterns in cloned embryos.Entities:
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Year: 2004 PMID： 15581618 DOI： 10.1016/j.febslet.2004.10.073
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124