Literature DB >> 15574959

Flow cytometry-assisted cloning of specific sequence motifs from complex 16S rRNA gene libraries.

Jeppe L Nielsen1, Andreas Schramm, Anne E Bernhard, Gerrit J van den Engh, David A Stahl.   

Abstract

A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

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Year:  2004        PMID： 15574959      PMCID： PMC535210          DOI： 10.1128/AEM.70.12.7550-7554.2004

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

1.  High bacterial diversity in permanently cold marine sediments.

Authors:  K Ravenschlag; K Sahm; J Pernthaler; R Amann
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

2.  Changes in community composition during dilution cultures of marine bacterioplankton as assessed by flow cytometric and molecular biological techniques.

Authors:  B M Fuchs; M V Zubkov; K Sahm; P H Burkill; R Amann
Journal:  Environ Microbiol       Date:  2000-04       Impact factor: 5.491

3.  Fluorescence in situ hybridization of 16S rRNA gene clones (Clone-FISH) for probe validation and screening of clone libraries.

Authors:  Andreas Schramm; Bernhard M Fuchs; Jeppe L Nielsen; Mauro Tonolla; David A Stahl
Journal:  Environ Microbiol       Date:  2002-11       Impact factor: 5.491

4.  Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis: implications for molecular diversity surveys.

Authors:  U Purkhold; A Pommerening-Röser; S Juretschko; M C Schmid; H P Koops; M Wagner
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

5.  Optimization of single-base-pair mismatch discrimination in oligonucleotide microarrays.

Authors:  Hidetoshi Urakawa; Said El Fantroussi; Hauke Smidt; James C Smoot; Erik H Tribou; John J Kelly; Peter A Noble; David A Stahl
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

Review 6.  Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity.

Authors:  P Hugenholtz; B M Goebel; N R Pace
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

Review 7.  Bacterial phylogeny based on comparative sequence analysis.

Authors:  W Ludwig; O Strunk; S Klugbauer; N Klugbauer; M Weizenegger; J Neumaier; M Bachleitner; K H Schleifer
Journal:  Electrophoresis       Date:  1998-04       Impact factor: 3.535

Review 8.  A molecular view of microbial diversity and the biosphere.

Authors:  N R Pace
Journal:  Science       Date:  1997-05-02       Impact factor: 47.728

9.  Phylogenetic relationships of Thiomicrospira species and their identification in deep-sea hydrothermal vent samples by denaturing gradient gel electrophoresis of 16S rDNA fragments.

Authors:  G Muyzer; A Teske; C O Wirsen; H W Jannasch
Journal:  Arch Microbiol       Date:  1995-09       Impact factor: 2.552

10.  Flow sorting of microorganisms for molecular analysis.

Authors:  G Wallner; B Fuchs; S Spring; W Beisker; R Amann
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792
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  2 in total

1.  Pig manure contamination marker selection based on the influence of biological treatment on the dominant fecal microbial groups.

Authors:  Romain Marti; Patrick Dabert; Anne-Marie Pourcher
Journal:  Appl Environ Microbiol       Date:  2009-06-12       Impact factor: 4.792

2.  Finding the needles in the metagenome haystack.

Authors:  George A Kowalchuk; Arjen G C L Speksnijder; Kun Zhang; Robert M Goodman; Johannes A van Veen
Journal:  Microb Ecol       Date:  2007-03-08       Impact factor: 4.552
  2 in total

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