Literature DB >> 15564669

Purification and characterization of alpha-keto amide reductase from Saccharomyces cerevisiae.

Kohji Ishihara1, Hiroaki Yamamoto, Kazuya Mitsuhashi, Kazuyoshi Nishikawa, Sadao Tsuboi, Hideaki Tsuji, Nobuyoshi Nakajima.   

Abstract

An NADPH-dependent alpha-keto amide reductase was purified from Saccharomyces cerevisiae. The molecular mass of the native enzyme was estimated to be 33 and 36 kDa by gel filtration chromatography and SDS-polyacrylamide gel electrophoresis, respectively. The purified enzyme showed a reducing activity not only for aromatic alpha-keto amides but also for aliphatic and aromatic alpha-keto esters. The internal sequence of the enzyme was identical with that of a hypothetical protein (ORF YDL 124w) coded by yeast chromosome IV.

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Year:  2004        PMID： 15564669     DOI： 10.1271/bbb.68.2306

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  6 in total

1.  Resistance of Saccharomyces cerevisiae to high concentrations of furfural is based on NADPH-dependent reduction by at least two oxireductases.

Authors:  Dominik Heer; Daniel Heine; Uwe Sauer
Journal:  Appl Environ Microbiol       Date:  2009-10-23       Impact factor: 4.792

2.  Candidate target genes for the Saccharomyces cerevisiae transcription factor, Yap2.

Authors:  Seo Young Bang; Jeong Hoon Kim; Phil Young Lee; Seung-Wook Chi; Sayeon Cho; Gwan-Su Yi; Pyung Keun Myung; Byoung Chul Park; Kwang-Hee Bae; Sung Goo Park
Journal:  Folia Microbiol (Praha)       Date:  2013-01-19       Impact factor: 2.099

3.  Functional studies of aldo-keto reductases in Saccharomyces cerevisiae.

Authors:  Qing Chang; Terry A Griest; Theresa M Harter; J Mark Petrash
Journal:  Biochim Biophys Acta       Date:  2006-10-21

4.  A novel carbonyl reductase with anti-Prelog stereospecificity for the production of t-butyl 6-cyano-(3R, 5R)-dihydroxyhexanoate.

Authors:  Qingchao Jin; Zhige Wu; Yanping Dou; Yu Yang; Jingjing Xia; Zhihua Jin
Journal:  3 Biotech       Date:  2019-05-02       Impact factor: 2.406

5.  Disruption of aldo-keto reductase genes leads to elevated markers of oxidative stress and inositol auxotrophy in Saccharomyces cerevisiae.

Authors:  Qing Chang; J Mark Petrash
Journal:  Biochim Biophys Acta       Date:  2007-09-05

6.  Rh(I)-catalyzed intermolecular hydroacylation: enantioselective cross-coupling of aldehydes and ketoamides.

Authors:  Kevin G M Kou; Diane N Le; Vy M Dong
Journal:  J Am Chem Soc       Date:  2014-06-17       Impact factor: 15.419
  6 in total

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