Literature DB >> 1555993

Primer-dependent amplification of mdr1 mRNA by polymerase chain reaction.

I Sugawara1, M Watanabe, A Masunaga, S Itoyama, K Ueda.   

Abstract

We have developed a sensitive reverse transcription (RT)-polymerase chain reaction (PCR) to evaluate multidrug resistance in various tissues, using seven mdr-specific primer sets. mdr1 mRNA expression was noted in 16/197 samples. However, the apparent degree of mdr1 mRNA expression depended on the sequence of the primers employed. These findings suggest that more than two sets of primers should be used for effective RT-PCR.

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Year:  1992        PMID： 1555993      PMCID： PMC5918777          DOI： 10.1111/j.1349-7006.1992.tb00076.x

Source DB:  PubMed          Journal:  Jpn J Cancer Res        ISSN： 0910-5050


  15 in total

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Authors:  I Pastan; M Gottesman
Journal:  N Engl J Med       Date:  1987-05-28       Impact factor: 91.245

2.  Altered surface membrane glycoproteins in Vinca alkaloid-resistant human leukemic lymphoblasts.

Authors:  W T Beck; T J Mueller; L R Tanzer
Journal:  Cancer Res       Date:  1979-06       Impact factor: 12.701

3.  Sequence of mdr3 cDNA encoding a human P-glycoprotein.

Authors:  A M van der Bliek; P M Kooiman; C Schneider; P Borst
Journal:  Gene       Date:  1988-11-30       Impact factor: 3.688

4.  Detection of P-glycoprotein in ovarian cancer: a molecular marker associated with multidrug resistance.

Authors:  D R Bell; J H Gerlach; N Kartner; R N Buick; V Ling
Journal:  J Clin Oncol       Date:  1985-03       Impact factor: 44.544

Review 5.  Expression and functions of P-glycoprotein (mdr1 gene product) in normal and malignant tissues.

Authors:  I Sugawara
Journal:  Acta Pathol Jpn       Date:  1990-08

6.  Quantitative analysis of MDR1 (multidrug resistance) gene expression in human tumors by polymerase chain reaction.

Authors:  K E Noonan; C Beck; T A Holzmayer; J E Chin; J S Wunder; I L Andrulis; A F Gazdar; C L Willman; B Griffith; D D Von Hoff; I B Roninson
Journal:  Proc Natl Acad Sci U S A       Date:  1990-09       Impact factor: 11.205

7.  Detection of a multidrug resistant phenotype in acute non-lymphoblastic leukaemia.

Authors:  D D Ma; R D Scurr; R A Davey; S M Mackertich; D H Harman; G Dowden; J P Isbister; D R Bell
Journal:  Lancet       Date:  1987-01-17       Impact factor: 79.321

8.  Immunohistochemical detection of P-glycoprotein: prognostic correlation in soft tissue sarcoma of childhood.

Authors:  H S Chan; P S Thorner; G Haddad; V Ling
Journal:  J Clin Oncol       Date:  1990-04       Impact factor: 44.544

9.  P-glycoprotein expression in treated and untreated human breast cancer.

Authors:  J Schneider; M Bak; T Efferth; M Kaufmann; J Mattern; M Volm
Journal:  Br J Cancer       Date:  1989-12       Impact factor: 7.640

10.  Characterization of an etoposide-resistant human K562 cell line, K/eto.

Authors:  I Sugawara; T Iwahashi; K Okamoto; Y Sugimoto; H Ekimoto; T Tsuruo; T Ikeuchi; S Mori
Journal:  Jpn J Cancer Res       Date:  1991-09
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  3 in total

1.  Reduced nm 23-H1 messenger RNA expression in metastatic lymph nodes from patients with papillary carcinoma of the thyroid.

Authors:  T Arai; M Watanabe; M Onodera; T Yamashita; A Masunaga; S Itoyama; K Itoh; I Sugawara
Journal:  Am J Pathol       Date:  1993-06       Impact factor: 4.307

2.  Reversal effect of itraconazole on adriamycin and etoposide resistance in human leukemia cells.

Authors:  M Kurosawa; M Okabe; N Hara; K Kawamura; S Suzuki; K Sakurada; M Asaka
Journal:  Ann Hematol       Date:  1996-01       Impact factor: 3.673

3.  Preparation and characterization of a murine monoclonal antibody (MDR3M) reactive with mdr3 gene product.

Authors:  I Sugawara; A Hirata; K Ueda; S Itoyama
Journal:  Jpn J Cancer Res       Date:  1992-08
  3 in total

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