| Literature DB >> 15553149 |
Sabria Aued-Pimentel1, João Henrique Ghilardi Lago, Mariana Helena Chaves, Edna Emy Kumagai.
Abstract
Cyclopropenoids fatty acids (CPFA) from Sterculia striata seed oil were characterized by gas chromatography/mass spectrometry (GC/MS) and quantified by gas chromatography-flame ionization detector (GC-FID) after derivation to fatty acid methyl esters using a cold base-catalyzed procedure. 1H nuclear magnetic resonance (NMR) analysis were done in oil and fatty acid methyl esters derivatives to quantify CPFA and verify artifacts formation during the base-catalyzed reaction. Similar quantities of CPFA were found in S. striata and Sterculia foetida seed oils before and after a base-catalyzed methylation by NMR analysis, with no artifact formation. These results were compatible with those obtained by GC-FID analysis. Transmethylation with KOH in methanol was an appropriated method to prepare cyclopropenoids fatty acids methyl esters and quantify them by GC and NMR analysis.Entities:
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Year: 2004 PMID： 15553149 DOI： 10.1016/j.chroma.2004.07.090
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759