Literature DB >> 15547985

General method for suppression of diagonal peaks in heteronuclear-edited NOESY spectroscopy.

Jihui Wu1, Jing-song Fan, Steven M Pascal, Daiwen Yang.   

Abstract

A novel approach is proposed for suppression of diagonal peaks in 15N- and 13C-edited NOESY spectroscopy based on subtracting a spectrum with only diagonal peaks from the conventional NOESY spectrum. This method can be applied to most heteronuclear-edited NOESY experiments. It is far more sensitive than the TROSY-based approach for biomolecules with little TROSY effect, and nearly complete suppression of diagonal peaks can be achieved. The method has been demonstrated on samples of 15N-labeled calmodulin (17 kDa), 13C-labeled DdCAD-1 (24 kDa), and 13C-labeled hemoglobin (65 kDa), showing that cross-peaks very close to diagonals can be assigned reliably based on the difference spectra.

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Year:  2004        PMID： 15547985     DOI： 10.1021/ja045300l

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  10 in total

1.  Protein backbone dynamics through 13C'-13Calpha cross-relaxation in NMR spectroscopy.

Authors:  Fabien Ferrage; Philippe Pelupessy; David Cowburn; Geoffrey Bodenhausen
Journal:  J Am Chem Soc       Date:  2006-08-30       Impact factor: 15.419

2.  Solution structure of eggcase silk protein and its implications for silk fiber formation.

Authors:  Zhi Lin; Weidong Huang; Jingfeng Zhang; Jing-Song Fan; Daiwen Yang
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-20       Impact factor: 11.205

3.  Conformational analysis by quantitative NOE measurements of the β-proton pairs across individual disulfide bonds in proteins.

Authors:  Mitsuhiro Takeda; Tsutomu Terauchi; Masatsune Kainosho
Journal:  J Biomol NMR       Date:  2011-12-01       Impact factor: 2.835

4.  Fast acquisition of high resolution 4-D amide-amide NOESY with diagonal suppression, sparse sampling and FFT-CLEAN.

Authors:  Jon W Werner-Allen; Brian E Coggins; Pei Zhou
Journal:  J Magn Reson       Date:  2010-02-21       Impact factor: 2.229

5.  Efficient acquisition of high-resolution 4-D diagonal-suppressed methyl-methyl NOESY for large proteins.

Authors:  Jie Wen; Pei Zhou; Jihui Wu
Journal:  J Magn Reson       Date:  2012-03-09       Impact factor: 2.229

6.  Selective diagonal-free (13)C, (13)C-edited aliphatic-aromatic NOESY experiment with non-uniform sampling.

Authors:  Jan Stanek; Michał Nowakowski; Saurabh Saxena; Katarzyna Ruszczyńska-Bartnik; Andrzej Ejchart; Wiktor Koźmiński
Journal:  J Biomol NMR       Date:  2013-05-09       Impact factor: 2.835

7.  Solid-state NMR structure determination from diagonal-compensated, sparsely nonuniform-sampled 4D proton-proton restraints.

Authors:  Rasmus Linser; Benjamin Bardiaux; Loren B Andreas; Sven G Hyberts; Vanessa K Morris; Guido Pintacuda; Margaret Sunde; Ann H Kwan; Gerhard Wagner
Journal:  J Am Chem Soc       Date:  2014-07-25       Impact factor: 15.419

8.  Solution structure of Domains IVa and V of the tau subunit of Escherichia coli DNA polymerase III and interaction with the alpha subunit.

Authors:  Xun-Cheng Su; Slobodan Jergic; Max A Keniry; Nicholas E Dixon; Gottfried Otting
Journal:  Nucleic Acids Res       Date:  2007-04-22       Impact factor: 16.971

9.  Unusual architecture of the p7 channel from hepatitis C virus.

Authors:  Bo OuYang; Shiqi Xie; Marcelo J Berardi; Xinhao Zhao; Jyoti Dev; Wenjing Yu; Bing Sun; James J Chou
Journal:  Nature       Date:  2013-06-05       Impact factor: 49.962

10.  A general method for diagonal peak suppression in homonuclear correlated NMR spectra by spatially and frequency selective pulses.

Authors:  Simon Glanzer; Evelyne Schrank; Klaus Zangger
Journal:  J Magn Reson       Date:  2013-04-22       Impact factor: 2.229
  10 in total

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