| Literature DB >> 15547291 |
Sam J Barkley1, Shrivallabh B Desai, C Dale Poulter.
Abstract
Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His(6)-tagged protein was produced in Escherichia coli and purified by Ni(2+) chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg(2+) for activity; K(m)(IPP) was 52 microM, and k(cat)(IPP) was 0.23 s(-1).Entities:
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Year: 2004 PMID: 15547291 PMCID: PMC529070 DOI: 10.1128/JB.186.23.8156-8158.2004
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490