Literature DB >> 15537669

Selection of optical biosensors from chemisynthetic antibody libraries.

Laurent Jespers1, Timothy P Bonnert, Greg Winter.   

Abstract

We describe a method for creating antibodies with a fluorescent reporter integrated into the antigen-binding site. A reporter molecule was chemically linked to a hypervariable loop of an antibody repertoire displayed on phage, and this repertoire was selected for antigen binding. In one selected antibody, the fluorescence of the probe responded quantitatively to antigen binding. The method may have application for the engineering of homogeneous immunoassays.

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Year:  2004        PMID： 15537669     DOI： 10.1093/protein/gzh083

Source DB:  PubMed          Journal:  Protein Eng Des Sel        ISSN： 1741-0126            Impact factor:   1.650


  9 in total

1.  Selection of Color-Changing and Intensity-Increasing Fluorogenic Probe as Protein-Specific Indicator Obtained via the 10BASE(d)-T.

Authors:  Masumi Taki; Hiroaki Inoue; Kazuto Mochizuki; Jay Yang; Yuji Ito
Journal:  Anal Chem       Date:  2016-01-06       Impact factor: 6.986

2.  Design Principles for SuCESsFul Biosensors: Specific Fluorophore/Analyte Binding and Minimization of Fluorophore/Scaffold Interactions.

Authors:  Seymour de Picciotto; Paige M Dickson; Michael W Traxlmayr; Bryan S Marques; Elke Socher; Sixing Zhao; Stephanie Cheung; Jonathan D Kiefer; A Joshua Wand; Linda G Griffith; Barbara Imperiali; K Dane Wittrup
Journal:  J Mol Biol       Date:  2016-07-21       Impact factor: 5.469

3.  Phage-encoded combinatorial chemical libraries based on bicyclic peptides.

Authors:  Christian Heinis; Trevor Rutherford; Stephan Freund; Greg Winter
Journal:  Nat Chem Biol       Date:  2009-07       Impact factor: 15.040

4.  A polar ring endows improved specificity to an antibody fragment.

Authors:  Zachary P Schaefer; Lucas J Bailey; Anthony A Kossiakoff
Journal:  Protein Sci       Date:  2016-03-09       Impact factor: 6.725

5.  Engineered protein-small molecule conjugates empower selective enzyme inhibition.

Authors:  Andrew K Lewis; Abbigael Harthorn; Sadie M Johnson; Roy R Lobb; Benjamin J Hackel
Journal:  Cell Chem Biol       Date:  2021-08-06       Impact factor: 8.116

6.  Chemical Diversification of Simple Synthetic Antibodies.

Authors:  Mariha Islam; Haixing P Kehoe; Jacob B Lissoos; Manjie Huang; Christopher E Ghadban; Greg Berumen Sánchez; Hanan Z Lane; James A Van Deventer
Journal:  ACS Chem Biol       Date:  2021-01-22       Impact factor: 5.100

7.  Expanding the Chemical Diversity of Genetically Encoded Libraries.

Authors:  Sabrina E Iskandar; Victoria A Haberman; Albert A Bowers
Journal:  ACS Comb Sci       Date:  2020-11-09       Impact factor: 3.903

Review 8.  Chemical strategies for the covalent modification of filamentous phage.

Authors:  Jenna M L Bernard; Matthew B Francis
Journal:  Front Microbiol       Date:  2014-12-23       Impact factor: 5.640

9.  Ultra Q-bodies: quench-based antibody probes that utilize dye-dye interactions with enhanced antigen-dependent fluorescence.

Authors:  Ryoji Abe; Hee-Jin Jeong; Dai Arakawa; Jinhua Dong; Hiroyuki Ohashi; Rena Kaigome; Fujio Saiki; Kyosuke Yamane; Hiroaki Takagi; Hiroshi Ueda
Journal:  Sci Rep       Date:  2014-04-11       Impact factor: 4.379
  9 in total

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