Literature DB >> 15528756

An unexpected experimental pitfall in the molecular diagnosis of bacterial endophthalmitis.

Luana Ugahary1, Wendy van de Sande, Jan C van Meurs, Alex van Belkum.   

Abstract

General primer-mediated ribosomal DNA amplification during endophthalmitis may improve the quality of diagnostic microbiology. However, extreme care needs to be taken not to introduce contaminating bacterial DNA during surgery procedures. The use of decontaminating iodine solutions can lead to such contamination due to the presence of DNA from Pseudomonas-like organisms.

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Year:  2004        PMID： 15528756      PMCID： PMC525270          DOI： 10.1128/JCM.42.11.5403-5405.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Improved detection of microorganisms by polymerase chain reaction in delayed endophthalmitis after cataract surgery.

Authors:  C P Lohmann; H J Linde; U Reischl
Journal:  Ophthalmology       Date:  2000-06       Impact factor: 12.079

2.  Rapid and simple method for purification of nucleic acids.

Authors:  R Boom; C J Sol; M M Salimans; C L Jansen; P M Wertheim-van Dillen; J van der Noordaa
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

3.  An Australia-wide epidemic of Pseudomonas pickettii bacteraemia due to contaminated "sterile" water for injection.

Authors:  L A Roberts; P J Collignon; V B Cramp; S Alexander; A E McFarlane; E Graham; A Fuller; V Sinickas; A Hellyar
Journal:  Med J Aust       Date:  1990-06-18       Impact factor: 7.738

4.  Evaluation of microbiological diagnostic techniques in postoperative endophthalmitis in the Endophthalmitis Vitrectomy Study.

Authors:  M Barza; P R Pavan; B H Doft; S R Wisniewski; L A Wilson; D P Han; S F Kelsey
Journal:  Arch Ophthalmol       Date:  1997-09

5.  PCR-RFLP-mediated detection and speciation of bacterial species causing endophthalmitis.

Authors:  N Okhravi; P Adamson; M M Matheson; H M Towler; S Lightman
Journal:  Invest Ophthalmol Vis Sci       Date:  2000-05       Impact factor: 4.799

6.  PCR-based evidence of bacterial involvement in eyes with suspected intraocular infection.

Authors:  N Okhravi; P Adamson; N Carroll; A Dunlop; M M Matheson; H M Towler; S Lightman
Journal:  Invest Ophthalmol Vis Sci       Date:  2000-10       Impact factor: 4.799

7.  Nosocomial Pseudomonas cepacia infection associated with chlorhexidine contamination.

Authors:  J D Sobel; N Hashman; G Reinherz; D Merzbach
Journal:  Am J Med       Date:  1982-08       Impact factor: 4.965

8.  Infections and pseudoinfections due to povidone-iodine solution contaminated with Pseudomonas cepacia.

Authors:  A L Panlilio; C M Beck-Sague; J D Siegel; R L Anderson; S Y Yetts; N C Clark; P N Duer; K A Thomassen; R W Vess; B C Hill
Journal:  Clin Infect Dis       Date:  1992-05       Impact factor: 9.079

9.  Diagnosis of infectious endophthalmitis after cataract surgery by polymerase chain reaction.

Authors:  C P Lohmann; M Heeb; H J Linde; V P Gabel; U Reischl
Journal:  J Cataract Refract Surg       Date:  1998-06       Impact factor: 3.351

10.  Results of the Endophthalmitis Vitrectomy Study. A randomized trial of immediate vitrectomy and of intravenous antibiotics for the treatment of postoperative bacterial endophthalmitis. Endophthalmitis Vitrectomy Study Group.

Authors: 
Journal:  Arch Ophthalmol       Date:  1995-12
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  1 in total

1.  Validation of a multi-species-specific PCR panel to diagnose patients with suspected postoperative bacterial endophthalmitis.

Authors:  Justin van Halsema; Ruud Jansen; Adriaan Heineken; Tjaco M van Ossewaarde; Magda A Meester-Smoor; Jan C van Meurs
Journal:  Acta Ophthalmol       Date:  2021-07-13       Impact factor: 3.988
  1 in total

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