Literature DB >> 15522511

Rapid detection of Panton-Valentine leukocidin from clinical isolates of Staphylococcus aureus strains by real-time PCR.

Ruud H Deurenberg1, Cornelis Vink, Christel Driessen, Michèle Bes, Nancy London, Jerome Etienne, Ellen E Stobberingh.   

Abstract

To allow rapid identification of Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus strains, a real-time PCR assay for detection of PVL was developed. This assay is convenient, since it can be applied directly on bacterial suspensions and does not require previous DNA purification. Furthermore, the assay was found to be highly reproducible, robust and specific, since positive results were generated exclusively with PVL-positive S. aureus strains, and neither with PVL-negative strains nor staphylococci other than S. aureus.

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Year:  2004        PMID： 15522511     DOI： 10.1016/j.femsle.2004.09.031

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN： 0378-1097            Impact factor:   2.742


  15 in total

1.  Different clonal complexes of methicillin-resistant Staphylococcus aureus are disseminated in the Euregio Meuse-Rhine region.

Authors:  Ruud H Deurenberg; Cornelis Vink; Guy J Oudhuis; Jascha E Mooij; Christel Driessen; Guy Coppens; Jos Craeghs; Els De Brauwer; Sebastian Lemmen; Hans Wagenvoort; Alexander W Friedrich; Jacques Scheres; Ellen E Stobberingh
Journal:  Antimicrob Agents Chemother       Date:  2005-10       Impact factor: 5.191

2.  Development of a triplex real-time PCR assay for detection of Panton-Valentine leukocidin toxin genes in clinical isolates of methicillin-resistant Staphylococcus aureus.

Authors:  Ryan R McDonald; Nick A Antonishyn; Toni Hansen; Laelie A Snook; Evelyn Nagle; Michael R Mulvey; Paul N Levett; Greg B Horsman
Journal:  J Clin Microbiol       Date:  2005-12       Impact factor: 5.948

3.  Staphylococcal cassette chromosome mec and Panton-Valentine leukocidin characterization of methicillin-resistant Staphylococcus aureus clones.

Authors:  Shannon M Moroney; Loree C Heller; Jesse Arbuckle; Monica Talavera; Ray H Widen
Journal:  J Clin Microbiol       Date:  2006-12-27       Impact factor: 5.948

4.  Rapid detection of Panton-Valentine leukocidin-positive Staphylococcus aureus by real-time PCR targeting the lukS-PV gene.

Authors:  U Reischl; M J Tuohy; G S Hall; G W Procop; N Lehn; H Linde
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-02       Impact factor: 3.267

5.  Novel multiplex PCR assay for detection of the staphylococcal virulence marker Panton-Valentine leukocidin genes and simultaneous discrimination of methicillin-susceptible from -resistant staphylococci.

Authors:  Jo-Ann McClure; John M Conly; Vicky Lau; Sameer Elsayed; Thomas Louie; Wendy Hutchins; Kunyan Zhang
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

6.  Genetic diversity of methicillin-resistant Staphylococcus aureus in a tertiary hospital in The Netherlands between 2002 and 2006.

Authors:  E Nulens; E E Stobberingh; E Smeets; H van Dessel; M A Welling; S Sebastian; F H van Tiel; P S Beisser; R H Deurenberg
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2009-01-08       Impact factor: 3.267

7.  Prevalence of community-associated meticillin-resistant Staphylococcus aureus and Panton-Valentine leucocidin-positive S. aureus in general practice patients with skin and soft tissue infections in the northern and southern regions of The Netherlands.

Authors:  D Mithoe; M I A Rijnders; B M Roede; E Stobberingh; A V M Möller
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2011-06-18       Impact factor: 3.267

8.  Successful management of Panton-Valentine leukocidine-positive necrotising pneumonia and A/H1N12009 influenzavirus coinfection in adult.

Authors:  Karena Riedweg-Moreno; Frederic Wallet; Caroline Blazejewski; Anne Goffard
Journal:  BMJ Case Rep       Date:  2014-01-16

9.  Population structure of Staphylococcus aureus strains isolated from intensive care unit patients in the netherlands over an 11-year period (1996 to 2006).

Authors:  M I A Rijnders; R H Deurenberg; M L L Boumans; J A A Hoogkamp-Korstanje; P S Beisser; E E Stobberingh
Journal:  J Clin Microbiol       Date:  2009-10-07       Impact factor: 5.948

10.  Cross-border dissemination of methicillin-resistant Staphylococcus aureus, Euregio Meuse-Rhin region.

Authors:  Ruud H Deurenberg; Eric Nulens; Havard Valvatne; Silvie Sebastian; Christel Driessen; Jos Craeghs; Els De Brauwer; Bernhard Heising; Yvette J Kraat; Joachim Riebe; Frans S Stals; Thera A Trienekens; Jacques Scheres; Alexander W Friedrich; Frank H van Tiel; Patrick S Beisser; Ellen E Stobberingh
Journal:  Emerg Infect Dis       Date:  2009-05       Impact factor: 6.883
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