OBJECTIVES: To investigate environmental contamination by extended-spectrum beta-lactamase-producing Enterobacteriaceae and to perform a comparative molecular analysis of clinical and environmental strains. SETTING: A 17-bed cardiac surgery intensive care unit of a 480-bed university teaching hospital. METHODS: Following an outbreak of extended-spectrum beta-lactamase-producing Enterobacteriaceae, an environmental survey revealed extensive contamination of the environment (particularly faucets, sink drains, and the joints of the countertops) by extended-spectrum beta-lactamase-producing Enterobacteriaceae. Environmental strains were compared with clinical strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA. RESULTS: A total of 62 environmental strains belonging to 4 species of extended-spectrum beta-lactamase-producing Enterobacteriaceae were analyzed and compared with 43 clinical strains obtained from 34 patients. Comparative molecular analysis revealed 4 identical or closely related patterns (3 from Klebsiella oxytoca and 1 from Enterobacter cloacae) between environmental and clinical strains. CONCLUSIONS: Moist surfaces may serve as sources of multiply resistant Enterobacteriaceae in the intensive care unit. Identification and disinfection of such sources may therefore be helpful in prevention and control of outbreaks.
OBJECTIVES: To investigate environmental contamination by extended-spectrum beta-lactamase-producing Enterobacteriaceae and to perform a comparative molecular analysis of clinical and environmental strains. SETTING: A 17-bed cardiac surgery intensive care unit of a 480-bed university teaching hospital. METHODS: Following an outbreak of extended-spectrum beta-lactamase-producing Enterobacteriaceae, an environmental survey revealed extensive contamination of the environment (particularly faucets, sink drains, and the joints of the countertops) by extended-spectrum beta-lactamase-producing Enterobacteriaceae. Environmental strains were compared with clinical strains by pulsed-field gel electrophoresis and randomly amplified polymorphic DNA. RESULTS: A total of 62 environmental strains belonging to 4 species of extended-spectrum beta-lactamase-producing Enterobacteriaceae were analyzed and compared with 43 clinical strains obtained from 34 patients. Comparative molecular analysis revealed 4 identical or closely related patterns (3 from Klebsiella oxytoca and 1 from Enterobacter cloacae) between environmental and clinical strains. CONCLUSIONS: Moist surfaces may serve as sources of multiply resistant Enterobacteriaceae in the intensive care unit. Identification and disinfection of such sources may therefore be helpful in prevention and control of outbreaks.
Authors: Adriana Manzur; Fe Tubau; Miquel Pujol; Laura Calatayud; Maria Angeles Dominguez; Carmen Peña; Mercedes Sora; Francesc Gudiol; Javier Ariza Journal: J Clin Microbiol Date: 2007-06-20 Impact factor: 5.948
Authors: Adebola O Ajao; J Kristie Johnson; Anthony D Harris; Min Zhan; Jessina C McGregor; Kerri A Thom; Jon P Furuno Journal: Infect Control Hosp Epidemiol Date: 2013-05 Impact factor: 3.254
Authors: Tom Van Maerken; Els De Brabandere; Audrey Noël; Liselotte Coorevits; Pascal De Waegemaeker; Raina Ablorh; Stefaan Bouchez; Ingrid Herck; Harlinde Peperstraete; Pierre Bogaerts; Bruno Verhasselt; Youri Glupczynski; Jerina Boelens; Isabel Leroux-Roels Journal: Antimicrob Resist Infect Control Date: 2019-09-18 Impact factor: 4.887