OBJECTIVE: To establish the quality criteria of the prepared slices of Paeonia lactiflon. METHOD: RP-HPLC was used for the determination of paeoniflorin in 10 lots of samples by ultrasound-assisted extraction. RESULT: The samples were extracted with 50% methanol. Seperation of the solution was performed on an ODS column with a mobile phase of acetonitrile-water (18:82), detected at 230 nm. CONCLUSION: The method is simple, repeatable, accurate and applicable.
OBJECTIVE: To establish the quality criteria of the prepared slices of Paeonia lactiflon. METHOD: RP-HPLC was used for the determination of paeoniflorin in 10 lots of samples by ultrasound-assisted extraction. RESULT: The samples were extracted with 50% methanol. Seperation of the solution was performed on an ODS column with a mobile phase of acetonitrile-water (18:82), detected at 230 nm. CONCLUSION: The method is simple, repeatable, accurate and applicable.