| Literature DB >> 15506247 |
Jukka-Pekka Suomela1, Markku Ahotupa, Olli Sjövall, Juha-Pekka Kurvinen, Heikki Kallio.
Abstract
The oxidation of human LDL lipids and the structures of oxidized TAG molecules found in LDL were investigated. Pooled samples of 10 normolipidemic and 10 hyperlipidemic subjects were analyzed. For determination of the oxidation levels, the LDL baseline diene conjugation (LDL-BDC) method was used. A method based on HPLC and electrospray ionization-MS was optimized and applied to the analysis of molecular structures of oxidized TAG in LDL. Differences were found between the oxidation levels of the samples. The LDL-BDC value was 22.2 micromol/L serum in the normolipidemic group, and 88.1 micromol/L serum in the hyperlipidemic group. The amounts of oxidized TAG molecules were small. However, several species of oxidized TAG were identified. These included TAG molecules with a keto or an epoxy group attached to a FA, and TAG molecules with a FA core aldehyde. In some TAG, the keto/epoxy ratio was greater in the hyperlipidemic group compared to the normolipidemic group. The results show that our approach is applicable to research on lipid oxidation in lipoproteins.Entities:
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Year: 2004 PMID： 15506247 DOI： 10.1007/s11745-004-1257-8
Source DB: PubMed Journal: Lipids ISSN： 0024-4201 Impact factor: 1.880