PURPOSE: To use progenitor cells isolated from the neural retina for transplantation studies in mice with retinal degeneration. METHODS: Retinal progenitor cells from postnatal day 1 green fluorescent protein-transgenic mice were isolated and characterized. These cells can be expanded greatly in culture and express markers characteristic of neural progenitor cells and/or retinal development. RESULTS: After they were grafted to the degenerating retina of mature mice, a subset of the retinal progenitor cells developed into mature neurons, including presumptive photoreceptors expressing recoverin, rhodopsin, or cone opsin. In rho-/- hosts, there was rescue of cells in the outer nuclear layer (ONL), along with widespread integration of donor cells into the inner retina, and recipient mice showed improved light-mediated behavior compared with control animals. CONCLUSIONS: These findings have implications for the treatment of retinal degeneration, in which neuronal replacement and photoreceptor rescue are major therapeutic goals.
PURPOSE: To use progenitor cells isolated from the neural retina for transplantation studies in mice with retinal degeneration. METHODS: Retinal progenitor cells from postnatal day 1 green fluorescent protein-transgenic mice were isolated and characterized. These cells can be expanded greatly in culture and express markers characteristic of neural progenitor cells and/or retinal development. RESULTS: After they were grafted to the degenerating retina of mature mice, a subset of the retinal progenitor cells developed into mature neurons, including presumptive photoreceptors expressing recoverin, rhodopsin, or cone opsin. In rho-/- hosts, there was rescue of cells in the outer nuclear layer (ONL), along with widespread integration of donor cells into the inner retina, and recipient mice showed improved light-mediated behavior compared with control animals. CONCLUSIONS: These findings have implications for the treatment of retinal degeneration, in which neuronal replacement and photoreceptor rescue are major therapeutic goals.
Authors: William L Neeley; Stephen Redenti; Henry Klassen; Sarah Tao; Tejal Desai; Michael J Young; Robert Langer Journal: Biomaterials Date: 2007-10-24 Impact factor: 12.479
Authors: David M Gamm; Lynda S Wright; Elizabeth E Capowski; Rebecca L Shearer; Jason S Meyer; Hyun-Jung Kim; Bernard L Schneider; John Nicholas Melvan; Clive N Svendsen Journal: Stem Cells Date: 2008-09-18 Impact factor: 6.277
Authors: Stewart Thompson; Frederick R Blodi; Swan Lee; Chris R Welder; Robert F Mullins; Budd A Tucker; Steven F Stasheff; Edwin M Stone Journal: Invest Ophthalmol Vis Sci Date: 2014-03-25 Impact factor: 4.799
Authors: Magdalene J Seiler; Robert B Aramant; Melissa K Jones; Dave L Ferguson; Elizabeth C Bryda; Hans S Keirstead Journal: Graefes Arch Clin Exp Ophthalmol Date: 2014-05-13 Impact factor: 3.117