Literature DB >> 15503814

Optimal conditions for the retrieval of CD4 and CD8 antigens in formalin-fixed, paraffin-embedded tissues.

Seok H Kim1, Myeong C Kook, Hyung G Song.   

Abstract

The effects of buffer, NaCl, EDTA, and urea on the retrieval of CD4 and CD8 antigens in formaldehyde-fixed, paraffin-embedded tissues with a microwave pressure-cooker were evaluated. The optimal retrieval conditions were found to be borate buffer at pH 8 containing 1 mM NaCl and 1 mM EDTA. Urea was found to be less effective.

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Year:  2004        PMID： 15503814     DOI： 10.1023/b:hijo.0000039856.43632.66

Source DB:  PubMed          Journal:  J Mol Histol        ISSN： 1567-2379            Impact factor:   2.611


  12 in total

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2.  The enhanced reactivity of endogenous biotin-like molecules by antigen retrieval procedures and signal amplification with tyramine.

Authors:  Seok Hyung Kim; Kyeong Cheon Jung; Young Kee Shin; Kyung Mee Lee; Young S Park; Yoon La Choi; Kwon Ik Oh; Min Kyung Kim; Doo Hyun Chung; Hyung Geun Son; Seong Hoe Park
Journal:  Histochem J       Date:  2002 Mar-Apr

3.  Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections.

Authors:  S R Shi; M E Key; K L Kalra
Journal:  J Histochem Cytochem       Date:  1991-06       Impact factor: 2.479

4.  Antigen unmasking on formalin-fixed, paraffin-embedded tissue sections.

Authors:  G Cattoretti; S Pileri; C Parravicini; M H Becker; S Poggi; C Bifulco; G Key; L D'Amato; E Sabattini; E Feudale
Journal:  J Pathol       Date:  1993-10       Impact factor: 7.996

5.  Use of pH 9.5 Tris-HCl buffer containing 5% urea for antigen retrieval immunohistochemistry.

Authors:  S R Shi; R J Cote; L Young; S A Imam; C R Taylor
Journal:  Biotech Histochem       Date:  1996-07       Impact factor: 1.718

6.  Comparative study of antigen retrieval heating methods: microwave, microwave and pressure cooker, autoclave, and steamer.

Authors:  C R Taylor; S R Shi; C Chen; L Young; C Yang; R J Cote
Journal:  Biotech Histochem       Date:  1996-09       Impact factor: 1.718

7.  New monoclonal antibodies to the T cell antigens CD4 and CD8. Production and characterization in formalin-fixed paraffin-embedded tissue.

Authors:  S L Williamson; M Steward; I Milton; A Parr; N H Piggott; A S Krajewski; B Angus; C H Horne
Journal:  Am J Pathol       Date:  1998-06       Impact factor: 4.307

8.  Hydrated autoclave pretreatment enhances tau immunoreactivity in formalin-fixed normal and Alzheimer's disease brain tissues.

Authors:  R W Shin; T Iwaki; T Kitamoto; J Tateishi
Journal:  Lab Invest       Date:  1991-05       Impact factor: 5.662

9.  Fixation, processing, and immunochemical reagent effects on preservation of T-lymphocyte surface membrane antigens in paraffin-embedded tissue.

Authors:  K Pollard; D Lunny; C S Holgate; P Jackson; C C Bird
Journal:  J Histochem Cytochem       Date:  1987-11       Impact factor: 2.479

10.  Antigen retrieval immunohistochemistry under the influence of pH using monoclonal antibodies.

Authors:  S R Shi; S A Imam; L Young; R J Cote; C R Taylor
Journal:  J Histochem Cytochem       Date:  1995-02       Impact factor: 2.479
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  4 in total

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Authors:  D Wang; C R Stockard; L Harkins; P Lott; C Salih; K Yuan; D Buchsbaum; A Hashim; M Zayzafoon; R W Hardy; O Hameed; W Grizzle; G P Siegal
Journal:  Biotech Histochem       Date:  2008-06       Impact factor: 1.718

2.  The transcription factor MIST1 is a novel human gastric chief cell marker whose expression is lost in metaplasia, dysplasia, and carcinoma.

Authors:  Jochen K M Lennerz; Seok-Hyung Kim; Edward L Oates; Won Jae Huh; Jason M Doherty; Xiaolin Tian; Andrew J Bredemeyer; James R Goldenring; Gregory Y Lauwers; Young-Kee Shin; Jason C Mills
Journal:  Am J Pathol       Date:  2010-08-13       Impact factor: 4.307

3.  Identification of 5-bromo-2'-deoxyuridine-labeled cells during mouse spermatogenesis by heat-induced antigen retrieval in lectin staining and immunohistochemistry.

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Journal:  J Histochem Cytochem       Date:  2014-12-05       Impact factor: 2.479

4.  Inhibition of Ca2+-independent phospholipase A2β (iPLA2β) ameliorates islet infiltration and incidence of diabetes in NOD mice.

Authors:  Robert N Bone; Ying Gai; Victoria Magrioti; Maroula G Kokotou; Tomader Ali; Xiaoyong Lei; Hubert M Tse; George Kokotos; Sasanka Ramanadham
Journal:  Diabetes       Date:  2014-09-11       Impact factor: 9.461
  4 in total

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