| Literature DB >> 15501807 |
Guiqing Wang1, Radha Iyer, Susan Bittker, Denise Cooper, Jennifer Small, Gary P Wormser, Ira Schwartz.
Abstract
The effects of variations in Barbour-Stoenner-Kelly (BSK) medium on the infectivity and pathogenicity of Borrelia burgdorferi clinical isolates were assessed by retrospective and prospective studies using a murine model of Lyme borreliosis. Thirty of 35 (86%) mice infected with any of six virulent B. burgdorferi clinical isolates grown in a BSK-H medium developed clinically apparent arthritis. By contrast, arthritis was observed in only 25 of 60 (42%) mice inoculated with two of these B. burgdorferi strains grown in a different lot of BSK-H medium (P < 0.001). In a prospective study, mice inoculated with a B. burgdorferi clinical isolate grown in a BSK medium prepared in-house produced significantly greater disease than those injected with the same isolate cultured in BSK-H medium (P < 0.05). The attenuated pathogenicity is not due to the loss of plasmids during in vitro cultivation. The data suggest that variations in BSK medium have a significant impact on the infectivity and pathogenicity of B. burgdorferi clinical isolates.Entities:
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Year: 2004 PMID： 15501807 PMCID： PMC523011 DOI： 10.1128/IAI.72.11.6702-6706.2004
Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441