Literature DB >> 15501033

Site-specific PEGylation of proteins containing unnatural amino acids.

Alexander Deiters1, T Ashton Cropp, Daniel Summerer, Mridul Mukherji, Peter G Schultz.   

Abstract

Here, we report a generally applicable PEGylation methodology based on the site-specific incorporation of para-azidophenylalanine into proteins in yeast. The azido group was used in a mild [3+2] cycloaddition reaction with an alkyne derivatized PEG reagent to afford selectively PEGylated protein. This strategy should be useful for the generation of selectively PEGylated proteins for therapeutic applications.

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Year:  2004        PMID： 15501033     DOI： 10.1016/j.bmcl.2004.09.059

Source DB:  PubMed          Journal:  Bioorg Med Chem Lett        ISSN： 0960-894X            Impact factor:   2.823


  48 in total

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2.  Alkyne- and 1,6-elimination- succinimidyl carbonate - terminated heterobifunctional poly(ethylene glycol) for reversible "Click" PEGylation.

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3.  Experimental methods for scanning unnatural amino acid mutagenesis.

Authors:  Jia Liu; T Ashton Cropp
Journal:  Methods Mol Biol       Date:  2012

4.  Tethered spectroscopic probes estimate dynamic distances with subnanometer resolution in voltage-dependent potassium channels.

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5.  Genetic encoding of non-natural amino acids in Drosophila melanogaster Schneider 2 cells.

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Review 6.  Applications of orthogonal "click" chemistries in the synthesis of functional soft materials.

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7.  'Clickable lectins': bioorthogonal reactive handles facilitate the directed conjugation of lectins in a modular fashion.

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Journal:  Interface Focus       Date:  2019-02-15       Impact factor: 3.906

8.  Conjugation Strategy Strongly Impacts the Conformational Stability of a PEG-Protein Conjugate.

Authors:  Paul B Lawrence; Wendy M Billings; McKenzie B Miller; Brijesh K Pandey; Andrew R Stephens; Minnie I Langlois; Joshua L Price
Journal:  ACS Chem Biol       Date:  2016-05-20       Impact factor: 5.100

9.  Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion.

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Journal:  Nucleic Acids Res       Date:  2010-02-16       Impact factor: 16.971

Review 10.  Synthetic biology approaches in drug discovery and pharmaceutical biotechnology.

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Journal:  Appl Microbiol Biotechnol       Date:  2010-04-16       Impact factor: 4.813
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