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Literature DB >> 154878

Some properties of vacuoles isolated from Neurospora crassa slime variant.

E Martinoia, U Heck, T Boller, A Wiemken, P Matile.

Abstract

A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total alpha-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.

Entities: Chemical Species

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Year: 1979 PMID： 154878 DOI： 10.1007/bf00413268

Source DB: PubMed Journal: Arch Microbiol ISSN： 0302-8933 Impact factor: 2.552

13 in total

1. [INTRACELLULAR LOCALIZATION OF PROTEOLYTIC ENZYMES OF NEUROSPORA CRASSA. I. FUNCTION AND SUBCELLULAR DISTRIBUTION OF PROTEOLYTIC ENZYMES].

Authors: P MATILE
Journal: Z Zellforsch Mikrosk Anat Date: 1965-03-16

Some properties of vacuoles isolated from Neurospora crassa slime variant.

1. [INTRACELLULAR LOCALIZATION OF PROTEOLYTIC ENZYMES OF NEUROSPORA CRASSA. I. FUNCTION AND SUBCELLULAR DISTRIBUTION OF PROTEOLYTIC ENZYMES].

2. Isolation of vacuoles from yeasts.

3. Polybase induced lysis of yeast spheroplasts. A new gentle method for preparation of vacuoles.

4. Proteolytic enzymes of Neurospora crassa. Purification and some properties of five intracellular proteinases.

5. Activities and cellular localization of yeast proteases and their inhibitors.

6. A particulate fraction from Neurospora crassa exhibiting aryl sulfatase activity.

7. A rapid, sensitive, and specific method for the determination of protein in dilute solution.

8. Intracellular localization of ornithine and arginine pools in Neurospora.

9. Characterization of a specific transport system for arginine in isolated yeast vacuoles.

10. Isolation of specific protease inhibitors from Neurospora crassa.

1. Purification of vacuoles from Neurospora crassa.

2. Isolation and Characterization of Vacuoles from the Ergot Fungus Claviceps purpurea.

3. Isolation and Characterization of Vacuoles from Melilotus alba Mesophyll.

Review 4. Chromosomal loci of Neurospora crassa.

5. Energetics of vacuolar compartmentation of arginine in Neurospora crassa.

6. Isolation and characterization of Neurospora crassa mutants impaired in feedback control of ornithine synthesis.

7. Purification of a vesicle-vacuole fraction functionally linked to aflatoxin synthesis in Aspergillus parasiticus.

8. Control of arginine metabolism in Neurospora: flux through the biosynthetic pathway.

9. Identification and properties of an ATPase in vacuolar membranes of Neurospora crassa.