| Literature DB >> 15481256 |
Anna Gliszczyńska-Swigło1, Ewa Sikorska.
Abstract
A simple and rapid reversed-phase high-performance liquid chromatography method for determination of alpha-, (beta + gamma), and delta-tocopherols in edible plant oils has been developed. Oils are diluted in 2-propanol and injected directly onto Symmetry C18 column. Methanol and acetonitrile (1:1) are used as a mobile phase. Tocopherols are detected using fluorescence detector set at excitation and emission wavelength 295 nm and 325 nm, respectively. The method is precise (R.S.D. not higher than 2.24%) and sensitive-detection limits (DL) are 8 ng/ml for gamma- and delta-tocopherols and 28 ng/ml for alpha-tocopherol; quantification limits (QL) were calculated as three times higher than DL.Entities:
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Year: 2004 PMID： 15481256 DOI： 10.1016/j.chroma.2004.07.051
Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759