OBJECTIVE: To determine in healthy children after primary infection the persistence of human herpesvirus 6 (HHV6) DNA, the presence and frequency of HHV6 re-activation or re-infection, and the relationship of both to illness and the presence of human herpesvirus 7 (HHV7) infection. STUDY DESIGN: Children 1 to 12 years of age with previous HHV6 infection were prospectively evaluated by HHV6 and HHV7 DNA polymerase chain reaction (PCR) and reverse transcription (RT)-PCR for HHV6. HHV6 plasma antibody titers were measured. Illnesses were recorded by diary, and physician records were reviewed. RESULTS: HHV6 DNA was detected in >or=1 peripheral blood mononuclear cell (PBMC) samples in 89% of children. HHV6 reactivation and re-infection were detected by RT-PCR in 1.1% of samples. Detection of HHV6 DNA was intermittent in 76% of children and was not associated with cumulative rates of illness. Illness at a study visit was significantly associated with the absence of HHV6 and HHV7 DNA in PBMC samples and was not associated with HHV6 antibody titer or the presence of HHV6 DNA in saliva. CONCLUSIONS: HHV6 DNA persists in most children intermittently following primary infection and is unrelated to illness. Reactivation of HHV6 occurs in healthy children without apparent illness.
OBJECTIVE: To determine in healthy children after primary infection the persistence of human herpesvirus 6 (HHV6) DNA, the presence and frequency of HHV6 re-activation or re-infection, and the relationship of both to illness and the presence of human herpesvirus 7 (HHV7) infection. STUDY DESIGN:Children 1 to 12 years of age with previous HHV6 infection were prospectively evaluated by HHV6 and HHV7 DNA polymerase chain reaction (PCR) and reverse transcription (RT)-PCR for HHV6. HHV6 plasma antibody titers were measured. Illnesses were recorded by diary, and physician records were reviewed. RESULTS:HHV6 DNA was detected in >or=1 peripheral blood mononuclear cell (PBMC) samples in 89% of children. HHV6 reactivation and re-infection were detected by RT-PCR in 1.1% of samples. Detection of HHV6 DNA was intermittent in 76% of children and was not associated with cumulative rates of illness. Illness at a study visit was significantly associated with the absence of HHV6 and HHV7 DNA in PBMC samples and was not associated with HHV6 antibody titer or the presence of HHV6 DNA in saliva. CONCLUSIONS:HHV6 DNA persists in most children intermittently following primary infection and is unrelated to illness. Reactivation of HHV6 occurs in healthy children without apparent illness.
Authors: Mary T Caserta; Caroline Breese Hall; Kenneth Schnabel; Geraldine Lofthus; Andrea Marino; Lynne Shelley; Christina Yoo; Jennifer Carnahan; Linda Anderson; Hongyue Wang Journal: J Clin Virol Date: 2010-03-07 Impact factor: 3.168
Authors: Katherine N Ward; Hoe Nam Leong; Elisabeth P Nacheva; Julie Howard; Claire E Atkinson; Nicholas W S Davies; Paul D Griffiths; Duncan A Clark Journal: J Clin Microbiol Date: 2006-04 Impact factor: 5.948
Authors: Caroline Breese Hall; Mary T Caserta; Kenneth C Schnabel; Lynne M Shelley; Jennifer A Carnahan; Andrea S Marino; Christina Yoo; Geraldine K Lofthus Journal: J Infect Dis Date: 2010-02-15 Impact factor: 5.226
Authors: Lynne M Mofenson; Michael T Brady; Susie P Danner; Kenneth L Dominguez; Rohan Hazra; Edward Handelsman; Peter Havens; Steve Nesheim; Jennifer S Read; Leslie Serchuck; Russell Van Dyke Journal: MMWR Recomm Rep Date: 2009-09-04
Authors: Leon G Epstein; Shlomo Shinnar; Dale C Hesdorffer; Douglas R Nordli; Aaliyah Hamidullah; Emma K T Benn; John M Pellock; L Matthew Frank; Darrell V Lewis; Solomon L Moshe; Ruth C Shinnar; Shumei Sun Journal: Epilepsia Date: 2012-06-14 Impact factor: 5.864