BACKGROUND: The overexpression of interferon (IFN)gamma or interleukin (IL)-13 in the adult murine lung induces the development of changes that mirror human lung emphysema. METHODS: IL-13 and IFNgamma expression was determined in lung samples from five groups of PATIENTS: severe emphysema without alpha(1)-antitrypsin deficiency (SE+, n = 10); severe emphysema with alpha(1)-antitrypsin deficiency (SE-, n = 5); mild localised emphysema (ME, n = 8); non-emphysema smokers (NE-S, n = 9), and non-emphysema non-smokers (NE-NS, n = 11). Lung IL-13 and IFNgamma mRNA were analysed by RT-PCR. Lung concentrations of IL-13 protein were assessed by ELISA. RESULTS: The expression of IFNgamma mRNA was similar in patients with or without emphysema. IL-13 mRNA was markedly decreased in the SE+ group compared with the SE- (p = 0.04), ME (p = 0.02), and non-emphysema groups (p = 0.01). IL-13 mRNA correlated with forced expiratory volume in 1 second (r = 0.5, p = 0.04) and arterial oxygen tension (r = 0.45, p = 0.03) in emphysema patients. In contrast to the non-emphysematous lung, IL-13 protein was below the detection limit of the assay in most emphysematous lung homogenates. CONCLUSION: The lung IL-13 content is reduced in patients with severe emphysema without alpha(1)-antitrypsin deficiency.
BACKGROUND: The overexpression of interferon (IFN)gamma or interleukin (IL)-13 in the adult murine lung induces the development of changes that mirror humanlung emphysema. METHODS:IL-13 and IFNgamma expression was determined in lung samples from five groups of PATIENTS: severe emphysema without alpha(1)-antitrypsin deficiency (SE+, n = 10); severe emphysema with alpha(1)-antitrypsin deficiency (SE-, n = 5); mild localised emphysema (ME, n = 8); non-emphysema smokers (NE-S, n = 9), and non-emphysema non-smokers (NE-NS, n = 11). Lung IL-13 and IFNgamma mRNA were analysed by RT-PCR. Lung concentrations of IL-13 protein were assessed by ELISA. RESULTS: The expression of IFNgamma mRNA was similar in patients with or without emphysema. IL-13 mRNA was markedly decreased in the SE+ group compared with the SE- (p = 0.04), ME (p = 0.02), and non-emphysema groups (p = 0.01). IL-13 mRNA correlated with forced expiratory volume in 1 second (r = 0.5, p = 0.04) and arterial oxygen tension (r = 0.45, p = 0.03) in emphysemapatients. In contrast to the non-emphysematous lung, IL-13 protein was below the detection limit of the assay in most emphysematous lung homogenates. CONCLUSION: The lung IL-13 content is reduced in patients with severe emphysema without alpha(1)-antitrypsin deficiency.
Authors: I Retamales; W M Elliott; B Meshi; H O Coxson; P D Pare; F C Sciurba; R M Rogers; S Hayashi; J C Hogg Journal: Am J Respir Crit Care Med Date: 2001-08-01 Impact factor: 21.405
Authors: T Zheng; Z Zhu; Z Wang; R J Homer; B Ma; R J Riese; H A Chapman; S D Shapiro; J A Elias Journal: J Clin Invest Date: 2000-11 Impact factor: 14.808
Authors: C G Lee; R J Homer; Z Zhu; S Lanone; X Wang; V Koteliansky; J M Shipley; P Gotwals; P Noble; Q Chen; R M Senior; J A Elias Journal: J Exp Med Date: 2001-09-17 Impact factor: 14.307
Authors: Hong Guo-Parke; Dermot Linden; Aurelie Mousnier; Ian C Scott; Helen Killick; Lee A Borthwick; Andrew J Fisher; Sinéad Weldon; Clifford C Taggart; Joseph C Kidney Journal: Front Med (Lausanne) Date: 2022-02-25
Authors: Phuwanat Sakornsakolpat; Dmitry Prokopenko; Brian D Hobbs; Michael H Cho; Maxime Lamontagne; Nicola F Reeve; Anna L Guyatt; Victoria E Jackson; Nick Shrine; Dandi Qiao; Traci M Bartz; Deog Kyeom Kim; Mi Kyeong Lee; Jeanne C Latourelle; Xingnan Li; Jarrett D Morrow; Ma'en Obeidat; Annah B Wyss; Per Bakke; R Graham Barr; Terri H Beaty; Steven A Belinsky; Guy G Brusselle; James D Crapo; Kim de Jong; Dawn L DeMeo; Tasha E Fingerlin; Sina A Gharib; Amund Gulsvik; Ian P Hall; John E Hokanson; Woo Jin Kim; David A Lomas; Stephanie J London; Deborah A Meyers; George T O'Connor; Stephen I Rennard; David A Schwartz; Pawel Sliwinski; David Sparrow; David P Strachan; Ruth Tal-Singer; Yohannes Tesfaigzi; Jørgen Vestbo; Judith M Vonk; Jae-Joon Yim; Xiaobo Zhou; Yohan Bossé; Ani Manichaikul; Lies Lahousse; Edwin K Silverman; H Marike Boezen; Louise V Wain; Martin D Tobin Journal: Nat Genet Date: 2019-02-25 Impact factor: 38.330