B Perez-Ordonez1, N N Huynh, K W Berean, R C K Jordan. 1. Department of Pathology, University Health Network, Princess Margaret Hospital, 610 University Avenue, Room 4-304, Toronto, Ontario M4N 3M5, Canada. perez-ordonez@uhn.on.ca
Abstract
BACKGROUND: Despite their histological resemblance to colorectal adenocarcinomas, there is little information about the molecular events involved in the pathogenesis of intestinal-type sinonasal adenocarcinomas (ITACs). AIMS: To evaluate the possible role of DNA mismatch repair (MMR) gene defects or disruptions of the E cadherin-beta catenin complex in ITAC by investigating the immunohistochemical expression of the MMR gene products, beta catenin, and E cadherin in a group of sporadic ITACs. METHODS: Ten sporadic cases of ITAC were stained with antibodies against MLH1, MSH2, MSH3, MSH6, beta catenin, and E cadherin. RESULTS: Nine cases showed strong nuclear expression of MLH1, whereas one case showed moderate staining. All 10 cases were strongly positive for MSH2 and MSH3. MSH6 was strong in nine cases, and moderate in one. Membranous beta catenin expression was strong in all 10 cases, and no case showed cytoplasmic or nuclear staining. E cadherin was strong in seven cases, and moderate in three cases. CONCLUSIONS: The preserved nuclear expression of MLH1, MSH2, MSH3, and MSH6 suggests that mutations or promoter methylation of MMR genes do not play a role in the pathogenesis of ITAC. The strong membranous staining for E cadherin and beta catenin and lack of abnormal cytoplasmic or nuclear expression is in keeping with the preservation of E cadherin-beta catenin complexes and beta catenin pathways.
BACKGROUND: Despite their histological resemblance to colorectal adenocarcinomas, there is little information about the molecular events involved in the pathogenesis of intestinal-type sinonasal adenocarcinomas (ITACs). AIMS: To evaluate the possible role of DNA mismatch repair (MMR) gene defects or disruptions of the E cadherin-beta catenin complex in ITAC by investigating the immunohistochemical expression of the MMR gene products, beta catenin, and E cadherin in a group of sporadic ITACs. METHODS: Ten sporadic cases of ITAC were stained with antibodies against MLH1, MSH2, MSH3, MSH6, beta catenin, and E cadherin. RESULTS: Nine cases showed strong nuclear expression of MLH1, whereas one case showed moderate staining. All 10 cases were strongly positive for MSH2 and MSH3. MSH6 was strong in nine cases, and moderate in one. Membranous beta catenin expression was strong in all 10 cases, and no case showed cytoplasmic or nuclear staining. E cadherin was strong in seven cases, and moderate in three cases. CONCLUSIONS: The preserved nuclear expression of MLH1, MSH2, MSH3, and MSH6 suggests that mutations or promoter methylation of MMR genes do not play a role in the pathogenesis of ITAC. The strong membranous staining for E cadherin and beta catenin and lack of abnormal cytoplasmic or nuclear expression is in keeping with the preservation of E cadherin-beta catenin complexes and beta catenin pathways.
Authors: Noralane M Lindor; Lawrence J Burgart; Olga Leontovich; Richard M Goldberg; Julie M Cunningham; Daniel J Sargent; Catherine Walsh-Vockley; Gloria M Petersen; Michael D Walsh; Barbara A Leggett; Joanne P Young; Melissa A Barker; Jeremy R Jass; John Hopper; Steve Gallinger; Bharati Bapat; Mark Redston; Stephen N Thibodeau Journal: J Clin Oncol Date: 2002-02-15 Impact factor: 44.544
Authors: Federica Perrone; Maria Oggionni; Sarah Birindelli; Simona Suardi; Silvia Tabano; Roberta Romano; Maria Luisa Moiraghi; Gabriella Bimbi; Pasquale Quattrone; Giulio Cantu; Marco A Pierotti; Lisa Licitra; Silvana Pilotti Journal: Int J Cancer Date: 2003-06-10 Impact factor: 7.396
Authors: J M D Wheeler; B F Warren; N J McC Mortensen; H C Kim; S C Biddolph; G Elia; N E Beck; G T Williams; N A Shepherd; A C Bateman; W F Bodmer Journal: Gut Date: 2002-02 Impact factor: 23.059
Authors: V A Marcus; L Madlensky; R Gryfe; H Kim; K So; A Millar; L K Temple; E Hsieh; T Hiruki; S Narod; B V Bapat; S Gallinger; M Redston Journal: Am J Surg Pathol Date: 1999-10 Impact factor: 6.394
Authors: Siobhan S Wahlberg; James Schmeits; George Thomas; Massimo Loda; Judy Garber; Sapna Syngal; Richard D Kolodner; Edward Fox Journal: Cancer Res Date: 2002-06-15 Impact factor: 12.701
Authors: Cristina García-Inclán; Fernando López; Jhudit Pérez-Escuredo; Mari Paz Cuesta-Albalad; Blanca Vivanco; Irene Centeno; Milagros Balbín; Carlos Suárez; José Luis Llorente; Mario A Hermsen Journal: Cell Oncol (Dordr) Date: 2012-10-11 Impact factor: 6.730
Authors: Jhudit Pérez-Escuredo; Jorge García Martínez; Cristina García-Inclán; Blanca Vivanco; María Costales; César Álvarez Marcos; José Luis Llorente; Mario A Hermsen Journal: Cell Oncol (Dordr) Date: 2011-01-18 Impact factor: 6.730
Authors: Paula Sánchez-Fernández; Cristina Riobello; María Costales; Blanca Vivanco; Virginia N Cabal; Rocío García-Marín; Laura Suárez-Fernández; Fernando López; Rubén Cabanillas; Mario A Hermsen; José Luis Llorente Journal: Sci Rep Date: 2021-01-26 Impact factor: 4.379