Literature DB >> 15449180

Rapid detection of Wuchereria bancrofti in mosquitoes by LightCycler polymerase chain reaction and melting curve analysis.

Virapong Lulitanond1, Pewpan M Intapan, Vichit Pipitgool, Wej Choochote, Wanchai Maleewong.   

Abstract

A LightCycler real-time polymerase chain reaction (PCR) assay was developed to detect Wuchereria bancrofti DNA in blood-fed mosquitoes. The assay is based on fluorescence melting curve analysis of the PCR product generated from a family of repeated DNA elements: the 182 bp SspI repeat, specific to the genus Wuchereria. According to the melting temperature, W. bancrofti infected-mosquitoes were differentiated from Brugia malayi-infected and non-infected mosquitoes as well as from genomic DNA of Dirofilaria immitis and human DNA. The method proved to be 100% sensitive in all W. bancrofti-infected mosquitoes. Melting curve analysis offers a rapid alternative for the specific detection of W. bancrofti in mosquitoes. It is very accurate and sensitive, allows a high throughput and can be performed on very small samples. The method therefore has great potential for application in epidemiological studies.

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Year:  2004        PMID： 15449180     DOI： 10.1007/s00436-004-1221-6

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  20 in total

Review 1.  PCR as a diagnostic and quantitative technique in veterinary parasitology.

Authors:  D S Zarlenga; J Higgins
Journal:  Vet Parasitol       Date:  2001-11-22       Impact factor: 2.738

2.  Global mapping of lymphatic filariasis.

Authors:  E Michael; D A Bundy
Journal:  Parasitol Today       Date:  1997-12

3.  Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction.

Authors:  O Kupferschmidt; D Krüger; T K Held; H Ellerbrok; W Siegert; K Janitschke
Journal:  Clin Microbiol Infect       Date:  2001-03       Impact factor: 8.067

4.  Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene.

Authors:  J Pietilä; Q He; J Oksi; M K Viljanen
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

5.  A polymerase chain reaction-based assay for detection of Wuchereria bancrofti in human blood and Culex pipiens.

Authors:  R M Ramzy; H A Farid; I H Kamal; G H Ibrahim; Z S Morsy; R Faris; G J Weil; S A Williams; A M Gad
Journal:  Trans R Soc Trop Med Hyg       Date:  1997 Mar-Apr       Impact factor: 2.184

6.  Scanning electron microscopic study of third-stage larva of Wuchereria bancrofti and Brugia malayi in Thailand.

Authors:  W Maleewong; W Choochote; K Sukhavat; C Khamboonruang; C Arunyanart
Journal:  Southeast Asian J Trop Med Public Health       Date:  1987-06       Impact factor: 0.267

7.  Detection of malaria liver-stages in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR.

Authors:  O Bruña-Romero; J C Hafalla; G González-Aseguinolaza; G Sano; M Tsuji; F Zavala
Journal:  Int J Parasitol       Date:  2001-11       Impact factor: 3.981

8.  Rapid differentiation of Old World Leishmania species by LightCycler polymerase chain reaction and melting curve analysis.

Authors:  Luc Nicolas; Geneviève Milon; Eric Prina
Journal:  J Microbiol Methods       Date:  2002-11       Impact factor: 2.363

9.  Effect of a single dose of primaquine on a Thai strain of Plasmodium falciparum.

Authors:  Y Chomcharn; K Surathin; D Bunnag; S Sucharit; T Harinasuta
Journal:  Southeast Asian J Trop Med Public Health       Date:  1980-09       Impact factor: 0.267

10.  A polymerase chain reaction assay for detection of the parasite Wuchereria bancrofti in human blood samples.

Authors:  M Zhong; J McCarthy; L Bierwert; M Lizotte-Waniewski; S Chanteau; T B Nutman; E A Ottesen; S A Williams
Journal:  Am J Trop Med Hyg       Date:  1996-04       Impact factor: 2.345
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  6 in total

1.  A real-time PCR-based assay for detection of Wuchereria bancrofti DNA in blood and mosquitoes.

Authors:  Ramakrishna U Rao; Laura J Atkinson; Reda M R Ramzy; Hanan Helmy; Hoda A Farid; Moses J Bockarie; Melinda Susapu; Sandra J Laney; Steven A Williams; Gary J Weil
Journal:  Am J Trop Med Hyg       Date:  2006-05       Impact factor: 2.345

2.  Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction.

Authors:  Jurairat Jongthawin; Pewpan M Intapan; Viraphong Lulitanond; Oranuch Sanpool; Tongjit Thanchomnang; Lakkhana Sadaow; Wanchai Maleewong
Journal:  Parasitol Res       Date:  2016-04-16       Impact factor: 2.289

3.  Molecular identification and phylogenetic analysis of Wuchereria bancrofti from human blood samples in Egypt.

Authors:  Iman R Abdel-Shafi; Eman Y Shoieb; Samar S Attia; José M Rubio; Thuy-Huong Ta-Tang; Ayman A El-Badry
Journal:  Parasitol Res       Date:  2017-01-24       Impact factor: 2.289

4.  Differential detection of Brugia malayi and Brugia pahangi by real-time fluorescence resonance energy transfer PCR and its evaluation for diagnosis of B. pahangi-infected dogs.

Authors:  Tongjit Thanchomnang; Pewpan M Intapan; Sudchit Chungpivat; Viraphong Lulitanond; Wanchai Maleewong
Journal:  Parasitol Res       Date:  2010-02       Impact factor: 2.289

5.  Detection of Opisthorchis viverrini in infected bithynid snails by real-time fluorescence resonance energy transfer PCR-based method and melting curve analysis.

Authors:  Pewpan M Intapan; Tongjit Thanchomnang; Viraphong Lulitanond; Phunthira Pongsaskulchoti; Wanchai Maleewong
Journal:  Parasitol Res       Date:  2008-05-30       Impact factor: 2.289

6.  Rapid detection and identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in mosquito vectors and blood samples by high resolution melting real-time PCR.

Authors:  Tongjit Thanchomnang; Pewpan M Intapan; Chairat Tantrawatpan; Viraphong Lulitanond; Sudchit Chungpivat; Piyanan Taweethavonsawat; Worasak Kaewkong; Oranuch Sanpool; Penchom Janwan; Wej Choochote; Wanchai Maleewong
Journal:  Korean J Parasitol       Date:  2013-12-31       Impact factor: 1.341
  6 in total

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