Literature DB >> 12223289

Rapid differentiation of Old World Leishmania species by LightCycler polymerase chain reaction and melting curve analysis.

Luc Nicolas1, Geneviève Milon, Eric Prina.   

Abstract

A LightCycler real-time polymerase chain reaction (PCR) assay has been developed to detect and differentiate four of the main Leishmania species of the Old World. The assay is based on fluorescence melting curve analysis of PCR products generated from the minicircles of kinetoplast DNA. According to the melting temperature, which is a function of GC/AT ratio, length and nucleotide sequences of the amplified product, Leishmania major was differentiated from L. donovani and from L. tropica and L. infantum. Melting curves analysis offers a rapid alternative for identification of species in diagnostic or epidemiological studies of leishmaniasis or asymptomatic parasitism. Copyright 2002 Elsevier Science B.V.

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Year:  2002        PMID： 12223289     DOI： 10.1016/s0167-7012(02)00099-4

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  40 in total

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Review 2.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

3.  Comparison of microscopic examination, rK39, and PCR for visceral leishmaniasis diagnosis in Turkey.

Authors:  Dilek Ozerdem; Fadime Eroglu; Ahmet Genc; Mehtap Demirkazik; Ismail Soner Koltas
Journal:  Parasitol Res       Date:  2009-10-27       Impact factor: 2.289

4.  Leishmania tropica in rock hyraxes (Procavia capensis) in a focus of human cutaneous leishmaniasis.

Authors:  Dalit Talmi-Frank; Charles L Jaffe; Abedelmajeed Nasereddin; Alon Warburg; Roni King; Milena Svobodova; Ofer Peleg; Gad Baneth
Journal:  Am J Trop Med Hyg       Date:  2010-05       Impact factor: 2.345

5.  Prevalence of bovine theileriosis in North Central region of Algeria by real-time polymerase chain reaction with a note on its distribution.

Authors:  Hocine Ziam; Rabah Kelanamer; Miriem Aissi; Assia Ababou; Dirk Berkvens; Dirk Geysen
Journal:  Trop Anim Health Prod       Date:  2015-04-01       Impact factor: 1.559

6.  Visceral leishmaniasis: a practical strategy for quantitative molecular diagnosis in naturally infected dogs.

Authors:  Sérgio Caldas; Andreza P Marcelino; Gilson Faria; Fernanda de Oliveira Silva; Ana Caroline Zampiroli Ataide; Lucas Maciel Cunha; Maria Terezinha Bahia; Gustavo Fontes Paz; Célia M F Gontijo
Journal:  Parasitol Res       Date:  2020-04-14       Impact factor: 2.289

7.  Detection and identification of Leishmania species from clinical specimens by using a real-time PCR assay and sequencing of the cytochrome B gene.

Authors:  Françoise Foulet; Françoise Botterel; Pierre Buffet; Gloria Morizot; Danièle Rivollet; Michèle Deniau; Francine Pratlong; Jean-Marc Costa; Stéphane Bretagne
Journal:  J Clin Microbiol       Date:  2007-05-02       Impact factor: 5.948

8.  Rapid detection of Wuchereria bancrofti in mosquitoes by LightCycler polymerase chain reaction and melting curve analysis.

Authors:  Virapong Lulitanond; Pewpan M Intapan; Vichit Pipitgool; Wej Choochote; Wanchai Maleewong
Journal:  Parasitol Res       Date:  2004-09-23       Impact factor: 2.289

9.  Comparison between quantitative nucleic acid sequence-based amplification, real-time reverse transcriptase PCR, and real-time PCR for quantification of Leishmania parasites.

Authors:  Wendy van der Meide; Jorge Guerra; Gerard Schoone; Marit Farenhorst; Leíla Coelho; William Faber; Inge Peekel; Henk Schallig
Journal:  J Clin Microbiol       Date:  2007-10-24       Impact factor: 5.948

10.  Development of a PCR Assay for Diagnosing Trematode (Opisthorchis and Haplorchis) Infections in Human Stools.

Authors:  Pheophet Lamaningao; Seiji Kanda; Sakhone Laimanivong; Takaki Shimono; Andrew Waleluma Darcy; Amphay Phyaluanglath; Nobuyuki Mishima; Toshimasa Nishiyama
Journal:  Am J Trop Med Hyg       Date:  2016-11-07       Impact factor: 2.345
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