Literature DB >> 15388966

Mode of AmyR binding to the CGGN8AGG sequence in the Aspergillus oryzae taaG2 promoter.

Tatsuo Ito1, Shuji Tani, Tomoyuki Itoh, Norihiro Tsukagoshi, Masashi Kato, Tetsuo Kobayashi.   

Abstract

AmyR is a transcriptional activator in Aspergillus spp. necessary for induction of the amylolytic enzyme genes. It recognizes 5'-CGGN8CGG-3' conserved in a number of the amylolytic gene promoters, and in addition 5'-CGGAAATTTAA-3' in the A. oryzae alpha-amylase promoter. In this report, interaction of AmyR with the 5'-CGGAAATTTAA-3' type binding site in the Taka-amylase gene (taaG2) promoter was precisely characterized by DNase I footprinting analysis and electrophoretic mobility shift assay in vitro, and also by examination of the in vivo activity of the mutated promoters. The in vitro and in vivo analyses indicated that two AmyR molecules bind cooperatively to the 5'-CGGAAATTTAA-3' sequence by recognizing the CGG triplet at the 5'-end and the AGG triplet just downstream of the sequence.

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Year:  2004        PMID： 15388966     DOI： 10.1271/bbb.68.1906

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  8 in total

1.  The Basic-Region Helix-Loop-Helix Transcription Factor DevR Significantly Affects Polysaccharide Metabolism in Aspergillus oryzae.

Authors:  Miao Zhuang; Zhi-Min Zhang; Long Jin; Bao-Teng Wang; Yasuji Koyama; Feng-Jie Jin
Journal:  Appl Environ Microbiol       Date:  2019-04-04       Impact factor: 4.792

Review 2.  Induction and Repression of Hydrolase Genes in Aspergillus oryzae.

Authors:  Mizuki Tanaka; Katsuya Gomi
Journal:  Front Microbiol       Date:  2021-05-24       Impact factor: 5.640

3.  Draft genome sequencing and comparative analysis of Aspergillus sojae NBRC4239.

Authors:  Atsushi Sato; Kenshiro Oshima; Hideki Noguchi; Masahiro Ogawa; Tadashi Takahashi; Tetsuya Oguma; Yasuji Koyama; Takehiko Itoh; Masahira Hattori; Yoshiki Hanya
Journal:  DNA Res       Date:  2011-06       Impact factor: 4.458

4.  Comparison and characterization of α-amylase inducers in Aspergillus nidulans based on nuclear localization of AmyR.

Authors:  Yuriko Murakoshi; Tomohiro Makita; Masashi Kato; Tetsuo Kobayashi
Journal:  Appl Microbiol Biotechnol       Date:  2012-01-18       Impact factor: 4.813

5.  A Robust Analytical Pipeline for Genome-Wide Identification of the Genes Regulated by a Transcription Factor: Combinatorial Analysis Performed Using gSELEX-Seq and RNA-Seq.

Authors:  Takaaki Kojima; Emi Kunitake; Kunio Ihara; Tetsuo Kobayashi; Hideo Nakano
Journal:  PLoS One       Date:  2016-07-13       Impact factor: 3.240

Review 6.  Regulators of plant biomass degradation in ascomycetous fungi.

Authors:  Tiziano Benocci; Maria Victoria Aguilar-Pontes; Miaomiao Zhou; Bernhard Seiboth; Ronald P de Vries
Journal:  Biotechnol Biofuels       Date:  2017-06-12       Impact factor: 6.040

7.  Identification of InuR, a new Zn(II)2Cys6 transcriptional activator involved in the regulation of inulinolytic genes in Aspergillus niger.

Authors:  Xiao-Lian Yuan; Johannes A Roubos; Cees A M J J van den Hondel; Arthur F J Ram
Journal:  Mol Genet Genomics       Date:  2007-10-05       Impact factor: 3.291

8.  Aspergillus niger genome-wide analysis reveals a large number of novel alpha-glucan acting enzymes with unexpected expression profiles.

Authors:  Xiao-Lian Yuan; Rachel M van der Kaaij; Cees A M J J van den Hondel; Peter J Punt; Marc J E C van der Maarel; Lubbert Dijkhuizen; Arthur F J Ram
Journal:  Mol Genet Genomics       Date:  2008-03-05       Impact factor: 3.291
  8 in total

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