Literature DB >> 15388734

Molecular analysis of artisanal Italian cheeses reveals Enterococcus italicus sp. nov.

M Grazia Fortina1, G Ricci1, D Mora1, P L Manachini1.   

Abstract

The taxonomic positions of seven atypical Enterococcus strains, isolated from artisanal Italian cheeses, were investigated in a polyphasic study. By using 16S rRNA gene sequencing, DNA-DNA hybridization and intergenic transcribed spacer analysis, as well as by examining the phenotypic properties, the novel isolates were shown to constitute a novel enterococcal species. Their closest relatives are Enterococcus sulfureus and Enterococcus saccharolyticus, having a 16S rRNA gene sequence similarity of 96.7 %. This group of strains can be easily differentiated from the other Enterococcus species by DNA-DNA hybridization and by their phenotypic characteristics: the strains do not grow in 6.5 % NaCl, and they do not produce acid from L-arabinose, melezitose, melibiose, raffinose or ribose. The name Enterococcus italicus sp. nov. is proposed for this species, with strain DSM 15952T (= LMG 22039T) as the type strain.

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Year:  2004        PMID: 15388734     DOI: 10.1099/ijs.0.63190-0

Source DB:  PubMed          Journal:  Int J Syst Evol Microbiol        ISSN: 1466-5026            Impact factor:   2.747


  9 in total

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2.  Phylogeny and identification of Enterococci by atpA gene sequence analysis.

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3.  Designation of the provisional new enterococcus species CDC PNS-E2 as Enterococcus sanguinicola sp. nov., isolated from human blood, and identification of a strain previously named Enterococcus CDC PNS-E1 as Enterococcus italicus Fortina, Ricci, Mora, and Manachini 2004.

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4.  Evaluation of plasmid content and tetracycline resistance conjugative transfer in Enterococcus italicus strains of dairy origin.

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9.  Genomic comparative analysis of the environmental Enterococcus mundtii against enterococcal representative species.

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  9 in total

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