| Literature DB >> 15388342 |
Katsuhito Hori1, Yuji Takahashi, Naoki Horikawa, Tetsushi Furukawa, Kazuhiro Tsukada, Noriaki Takeguchi, Hideki Sakai.
Abstract
It has been controversial whether the ClC-2 chloride channel is involved in hydrochloric acid secretion of gastric parietal cells. Here, we investigated whether ClC-2 is the apical Cl- channel associated with gastric acid secretion. Two anti-ClC-2 antibodies used in this study reacted with cloned ClC-2 protein expressed in HEK293 cells. In isolated rabbit gastric glands, significant expression of ClC-2 mRNA was observed, but the presence of ClC-2 protein was not clear. Furthermore, no expression of ClC-2 protein was observed in isolated rat and human gastric mucosa. Immunohistochemistry on the rat gastric mucosa showed no significant expression of ClC-2 protein in the parietal cells which showed abundant expression of H+,K+-ATPase. These results indicate that ClC-2 may not be a Cl- -transporting protein for gastric acid secretion in parietal cells.Entities:
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Year: 2004 PMID： 15388342 DOI： 10.1016/j.febslet.2004.08.044
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124