Literature DB >> 1538749

Sequence identification of 2,375 human brain genes.

M D Adams1, M Dubnick, A R Kerlavage, R Moreno, J M Kelley, T R Utterback, J W Nagle, C Fields, J C Venter.   

Abstract

We recently described a new approach for the rapid characterization of expressed genes by partial DNA sequencing to generate 'expressed sequence tags'. From a set of 600 human brain complementary DNA clones, 348 were informative nuclear-encoded messenger RNAs. We have now partially sequenced 2,672 new, independent cDNA clones isolated from four human brain cDNA libraries to generate 2,375 expressed sequence tags to nuclear-encoded genes. These sequences, together with 348 brain expressed sequence tags from our previous study, comprise more than 2,500 new human genes and 870,769 base pairs of DNA sequence. These data represent an approximate doubling of the number of human genes identified by DNA sequencing and may represent as many as 5% of the genes in the human genome.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1538749     DOI： 10.1038/355632a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  157 in total

1.  ExInt: an Exon/Intron database.

Authors:  M Sakharkar; M Long; T W Tan; S J de Souza
Journal:  Nucleic Acids Res       Date:  2000-01-01       Impact factor: 16.971

2.  Computer-based methods for the mouse full-length cDNA encyclopedia: real-time sequence clustering for construction of a nonredundant cDNA library.

Authors:  H Konno; Y Fukunishi; K Shibata; M Itoh; P Carninci; Y Sugahara; Y Hayashizaki
Journal:  Genome Res       Date:  2001-02       Impact factor: 9.043

3.  Shotgun sequencing of the human transcriptome with ORF expressed sequence tags.

Authors:  E Dias Neto; R G Correa; S Verjovski-Almeida; M R Briones; M A Nagai; W da Silva; M A Zago; S Bordin; F F Costa; G H Goldman; A F Carvalho; A Matsukuma; G S Baia; D H Simpson; A Brunstein; P S de Oliveira; P Bucher; C V Jongeneel; M J O'Hare; F Soares; R R Brentani; L F Reis; S J de Souza; A J Simpson
Journal:  Proc Natl Acad Sci U S A       Date:  2000-03-28       Impact factor: 11.205

4.  Expression profiling by iAFLP: A PCR-based method for genome-wide gene expression profiling.

Authors:  S Kawamoto; T Ohnishi; H Kita; O Chisaka; K Okubo
Journal:  Genome Res       Date:  1999-12       Impact factor: 9.043

5.  The pattern of gene expression in human CD15+ myeloid progenitor cells.

Authors:  S Lee; G Zhou; T Clark; J Chen; J D Rowley; S M Wang
Journal:  Proc Natl Acad Sci U S A       Date:  2001-03-13       Impact factor: 11.205

6.  Functional genomics in gastroenterology.

Authors:  S Schreiber; J Hampe; H Eickhoff; H Lehrach
Journal:  Gut       Date:  2000-11       Impact factor: 23.059

7.  Expression profiling across many samples via manifold-assisted mRNA processing.

Authors:  A Hagberg; G Barbany; H Krook; M Samiotaki; U Landegren
Journal:  Nucleic Acids Res       Date:  2000-06-01       Impact factor: 16.971

8.  A two-step strategy for constructing specifically self-subtracted cDNA libraries.

Authors:  Paolo Laveder; Cristiano De Pittà; Stefano Toppo; Giorgio Valle; Gerolamo Lanfranchi
Journal:  Nucleic Acids Res       Date:  2002-05-01       Impact factor: 16.971

Review 9.  Navigating the human transcriptome.

Authors:  R L Strausberg; G J Riggins
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-09       Impact factor: 11.205

10.  Molecular cloning and functional expression of a human cDNA encoding the antimutator enzyme 8-hydroxyguanine-DNA glycosylase.

Authors:  T Roldán-Arjona; Y F Wei; K C Carter; A Klungland; C Anselmino; R P Wang; M Augustus; T Lindahl
Journal:  Proc Natl Acad Sci U S A       Date:  1997-07-22       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.