| Literature DB >> 15381400 |
Abstract
Breaking a carbon-hydrogen bond adjacent to a carbonyl is a slow step in a large number of chemical reactions. However, many enzymes are capable of catalyzing this reaction with great efficiency. One of the most proficient of these enzymes is 3-oxo-Delta5-steroid isomerase (KSI), which catalyzes the isomerization of a wide variety of 3-oxo-Delta5-steroids to their Delta4-conjugated isomers. In this review, the mechanism of KSI is discussed, with particular emphasis on energetic considerations. Both experimental and theoretical approaches are considered to explain the mechanistic details of the reaction.Entities:
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Year: 2004 PMID： 15381400 DOI： 10.1016/j.bioorg.2004.06.005
Source DB: PubMed Journal: Bioorg Chem ISSN： 0045-2068 Impact factor: 5.275