| Literature DB >> 15380275 |
Gustavo H Goldman1, Márcia Eliana da Silva Ferreira, Everaldo dos Reis Marques, Marcela Savoldi, David Perlin, Steven Park, Patricio Christian Godoy Martinez, Maria Helena S Goldman, Arnaldo L Colombo.
Abstract
In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C. albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V437I, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C. albicans isolates from South America.Entities:
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Year: 2004 PMID： 15380275 DOI： 10.1016/j.diagmicrobio.2004.04.009
Source DB: PubMed Journal: Diagn Microbiol Infect Dis ISSN： 0732-8893 Impact factor: 2.803