Literature DB >> 28483951

The Etest Performed Directly on Blood Culture Bottles Is a Reliable Tool for Detection of Fluconazole-Resistant Candida albicans Isolates.

Pilar Escribano1,2, Laura Judith Marcos-Zambrano3,2, Ana Gómez3,2, Carlos Sánchez3,2,4, M Carmen Martínez-Jiménez3,2, Emilio Bouza3,2,4,5, Jesús Guinea1,2,4,5.   

Abstract

We assessed the ability of the Etest performed directly on positive blood cultures (ETDIR) to detect fluconazole susceptibility in 6 fluconazole-resistant and 12 fluconazole-susceptible Candida albicans isolates, according to CLSI M27-A3 and EUCAST EDef 7.2 procedures. Categorical agreement between ETDIR and broth microdilution was 100% when the trays were incubated at 25°C and trailing effect was ruled out. ETDIR is a reliable procedure when screening for the presence of fluconazole resistance in C. albicans.
Copyright © 2017 American Society for Microbiology.

Entities:  

Keywords:  Candida albicans; Etest; fluconazole; resistance

Mesh:

Substances:

Year:  2017        PMID: 28483951      PMCID: PMC5487615          DOI: 10.1128/AAC.00400-17

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN: 0066-4804            Impact factor:   5.191


  43 in total

1.  Time to initiation of fluconazole therapy impacts mortality in patients with candidemia: a multi-institutional study.

Authors:  Kevin W Garey; Milind Rege; Manjunath P Pai; Dana E Mingo; Katie J Suda; Robin S Turpin; David T Bearden
Journal:  Clin Infect Dis       Date:  2006-05-16       Impact factor: 9.079

2.  Trailing end-point phenotype of Candida spp. in antifungal susceptibility testing to fluconazole is eliminated by altering incubation temperature.

Authors:  D Agrawal; T F Patterson; M G Rinaldi; S G Revankar
Journal:  J Med Microbiol       Date:  2007-07       Impact factor: 2.472

3.  Optimizing the correlation between results of testing in vitro and therapeutic outcome in vivo for fluconazole by testing critical isolates in a murine model of invasive candidiasis.

Authors:  J H Rex; P W Nelson; V L Paetznick; M Lozano-Chiu; A Espinel-Ingroff; E J Anaissie
Journal:  Antimicrob Agents Chemother       Date:  1998-01       Impact factor: 5.191

4.  Antifungal susceptibility of 205 Candida spp. isolated primarily during invasive Candidiasis and comparison of the Vitek 2 system with the CLSI broth microdilution and Etest methods.

Authors:  N Bourgeois; L Dehandschoewercker; S Bertout; P-J Bousquet; P Rispail; L Lachaud
Journal:  J Clin Microbiol       Date:  2009-11-04       Impact factor: 5.948

5.  ESCMID* guideline for the diagnosis and management of Candida diseases 2012: non-neutropenic adult patients.

Authors:  O A Cornely; M Bassetti; T Calandra; J Garbino; B J Kullberg; O Lortholary; W Meersseman; M Akova; M C Arendrup; S Arikan-Akdagli; J Bille; E Castagnola; M Cuenca-Estrella; J P Donnelly; A H Groll; R Herbrecht; W W Hope; H E Jensen; C Lass-Flörl; G Petrikkos; M D Richardson; E Roilides; P E Verweij; C Viscoli; A J Ullmann
Journal:  Clin Microbiol Infect       Date:  2012-12       Impact factor: 8.067

6.  Delaying the empiric treatment of candida bloodstream infection until positive blood culture results are obtained: a potential risk factor for hospital mortality.

Authors:  Matthew Morrell; Victoria J Fraser; Marin H Kollef
Journal:  Antimicrob Agents Chemother       Date:  2005-09       Impact factor: 5.191

7.  Geographic variations in species distribution and echinocandin and azole antifungal resistance rates among Candida bloodstream infection isolates: report from the SENTRY Antimicrobial Surveillance Program (2008 to 2009).

Authors:  Michael A Pfaller; Gary J Moet; Shawn A Messer; Ronald N Jones; Mariana Castanheira
Journal:  J Clin Microbiol       Date:  2010-11-10       Impact factor: 5.948

8.  Comparison of antifungal MICs for yeasts obtained using the EUCAST method in a reference laboratory and the Etest in nine different hospital laboratories.

Authors:  E Dannaoui; A Paugam; M Develoux; C Chochillon; J Matheron; A Datry; C Bouges-Michel; C Bonnal; F Dromer; S Bretagne
Journal:  Clin Microbiol Infect       Date:  2009-09-23       Impact factor: 8.067

9.  Molecular identification and antifungal susceptibility of yeast isolates causing fungemia collected in a population-based study in Spain in 2010 and 2011.

Authors:  Jesús Guinea; Óscar Zaragoza; Pilar Escribano; Estrella Martín-Mazuelos; Javier Pemán; Ferrán Sánchez-Reus; Manuel Cuenca-Estrella
Journal:  Antimicrob Agents Chemother       Date:  2013-12-23       Impact factor: 5.191

10.  Distinct roles of Candida albicans drug resistance transcription factors TAC1, MRR1, and UPC2 in virulence.

Authors:  Andrea Lohberger; Alix T Coste; Dominique Sanglard
Journal:  Eukaryot Cell       Date:  2013-11-15
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  4 in total

1.  Clinical Characteristics and Outcomes of Candidemia Caused by Meyerozyma guilliermondii Complex in Cancer Patients Undergoing Surgery.

Authors:  Jingjing Chen; Sufei Tian; Fushun Li; Guoquan Sun; Ke Yun; Shitong Cheng; Yunzhuo Chu
Journal:  Mycopathologia       Date:  2020-09-28       Impact factor: 2.574

2.  Resistance to Echinocandins in Candida Can Be Detected by Performing the Etest Directly on Blood Culture Samples.

Authors:  Pilar Escribano; Jesús Guinea; María Ángeles Bordallo-Cardona; Laura Judith Marcos-Zambrano; Carlos Sánchez-Carrillo; Emilio Bouza; Patricia Muñoz
Journal:  Antimicrob Agents Chemother       Date:  2018-05-25       Impact factor: 5.191

3.  Direct Fluconazole Disk Susceptibility Testing for Candida glabrata-Positive Blood Cultures.

Authors:  Sarah Israel; Amichai Perlman; Jacob Moran-Gilad; Maya Korem
Journal:  J Clin Microbiol       Date:  2021-06-18       Impact factor: 5.948

Review 4.  Antifungal Susceptibly Testing by Concentration Gradient Strip Etest Method for Fungal Isolates: A Review.

Authors:  Eric Dannaoui; Ana Espinel-Ingroff
Journal:  J Fungi (Basel)       Date:  2019-11-22
  4 in total

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