OBJECTIVE: To identify genes involved in fibroid development by performing global expression profiling on tissues of normal myometrium and uterine leiomyoma origin using Affymetrix HG-U133A GeneChip microarrays. DESIGN: Whole-genome analysis of mRNA levels in leiomyoma and normal myometrium tissue samples. SETTING: University research laboratory. PATIENT(S): Eight patients of varying age and race undergoing surgery for symptomatic fibroids. INTERVENTION(S): After tissue collection of five tumors and five normals from human pathological specimens, labeled cRNA was generated and hybridized to the oligonucleotide-composed arrays. MAIN OUTCOME MEASURE(S): Quantification of transcript expression levels in uterine fibroids relative to normal myometrium. RESULT(S): Model-based expression analysis revealed that of the 22,500 transcripts represented on the arrays, 226 genes were found to be dysregulated by a > or =1.5-fold change between leiomyoma and normal myometrium. Moreover, our research identified many dysregulated apoptosis-related genes, of particular interest was TRAIL and Ask1, and also found numerous differentially expressed proliferation genes, including TGFB1, PDGFC, and two dual specificity phosphatases. CONCLUSION(S): These results indicate that these genes may play a significant role in the development of leiomyomas from normal uterine tissue. We hypothesize that the deregulation of apoptotic and proliferative processes is pivotal to fibroid development.
OBJECTIVE: To identify genes involved in fibroid development by performing global expression profiling on tissues of normal myometrium and uterine leiomyoma origin using Affymetrix HG-U133A GeneChip microarrays. DESIGN: Whole-genome analysis of mRNA levels in leiomyoma and normal myometrium tissue samples. SETTING: University research laboratory. PATIENT(S): Eight patients of varying age and race undergoing surgery for symptomatic fibroids. INTERVENTION(S): After tissue collection of five tumors and five normals from human pathological specimens, labeled cRNA was generated and hybridized to the oligonucleotide-composed arrays. MAIN OUTCOME MEASURE(S): Quantification of transcript expression levels in uterine fibroids relative to normal myometrium. RESULT(S): Model-based expression analysis revealed that of the 22,500 transcripts represented on the arrays, 226 genes were found to be dysregulated by a > or =1.5-fold change between leiomyoma and normal myometrium. Moreover, our research identified many dysregulated apoptosis-related genes, of particular interest was TRAIL and Ask1, and also found numerous differentially expressed proliferation genes, including TGFB1, PDGFC, and two dual specificity phosphatases. CONCLUSION(S): These results indicate that these genes may play a significant role in the development of leiomyomas from normal uterine tissue. We hypothesize that the deregulation of apoptotic and proliferative processes is pivotal to fibroid development.
Authors: Erica E Marsh; Marissa L Steinberg; J Brandon Parker; Ju Wu; Debabrata Chakravarti; Serdar E Bulun Journal: Fertil Steril Date: 2016-05-24 Impact factor: 7.329
Authors: Erica N Nierth-Simpson; Melvenia M Martin; Tung-Chin Chiang; Lilia I Melnik; Lyndsay V Rhodes; Shannon E Muir; Matthew E Burow; John A McLachlan Journal: Endocrinology Date: 2009-01-29 Impact factor: 4.736
Authors: Irina K Dimitrova; Jennifer K Richer; Michael C Rudolph; Nicole S Spoelstra; Elaine M Reno; Theresa M Medina; Andrew P Bradford Journal: Fertil Steril Date: 2008-07-30 Impact factor: 7.329
Authors: Pradeep S Tanwar; Ho-Joon Lee; LiHua Zhang; Lawrence R Zukerberg; Makoto M Taketo; Bo R Rueda; Jose M Teixeira Journal: Biol Reprod Date: 2009-04-29 Impact factor: 4.285