| Literature DB >> 1537401 |
D A Zelenina1, O I Kulaeva, E V Smirnyagina, A G Solovyev, N A Miroshnichenko, O N Fedorkin, N P Rodionova, J G Atabekov.
Abstract
The double-stranded DNA copy corresponding to the 5'-nontranslated alpha beta-leader of potato virus X (PVX) genomic RNA (positions -3 to-85 according to AUG initiator) was chemically synthesized and fused to the transcription plasmids containing three different reporter genes: neomycinphosphotransferase type II (NPT II) gene, Bacillus thuringiensis coleopteran-specific toxic protein gene and beta-glucuronidase (GUS) gene. Expression of the reporter genes in vitro and in plant protoplasts (in the case of GUS gene) reveals that the alpha beta-leader of PVX RNA acts as a translation enhancer despite the presence of the upstream vector-derived sequence and irrespective of the length of the spacer sequence preceding the reporter genes.Entities:
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Year: 1992 PMID： 1537401 DOI： 10.1016/0014-5793(92)80301-v
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124