| Literature DB >> 15369136 |
Hideki Harada1, Kaoru Saijo, Isamu Ishiwata, Tadao Ohno.
Abstract
An anchorage-dependent Wilms' tumor cell line, HFWT, has been found to be extremely susceptible to human natural killer (NK) cells. Here we established a transfectant of HFWT with the green fluorescence protein (GFP) gene, designated GHINK-1 cells, to apply to the activated NK cytotoxicity assay without radioisotope labeling. After being co-cultured with CD3 CD56+ NK cells, GHINK-1 cells released GFP into the medium. The intensity of the fluorescence from the released GFP correlated almost exactly with the radioactivity of a standard 51Cr-release assay performed with suspension-cultured K562 cells. Because it does not require separation of the remaining live target cells by centrifugation, the non-radioisotopic GFP release assay with GHINK-1 cells is a convenient alternative for monitoring human activated NK killing activity.Entities:
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Year: 2004 PMID： 15369136 DOI： 10.1111/j.1749-0774.2004.tb00019.x
Source DB: PubMed Journal: Hum Cell ISSN： 0914-7470 Impact factor: 4.174