OBJECTIVES: To investigate the mechanism of ciprofloxacin resistance in isolates of Acinetobacter baumannii during two hospital outbreaks and to determine the expression level of the gene encoding the AdeB efflux pump. METHODS: Isolates were previously typed by PFGE and their MICs determined by broth microdilution. The gyrA and parC genes were sequenced and the adeB gene examined by real-time reverse transcription PCR (RT-PCR). RESULTS: Two clonal lineages were responsible for the two hospital outbreaks. In both outbreaks, ciprofloxacin susceptibility was reduced during the course of the outbreak when compared with the index isolates. Mutations in gyrA and parC were found to have occurred during the outbreak. The MICs of non-fluoroquinolone antibiotics were raised in one clonal lineage and this was associated with a >10-fold increase in mRNA transcripts for adeB. CONCLUSIONS: We have witnessed the appearance of gyrA and parC mutations during outbreaks of A. baumannii. In parallel with these mutations, we observed up-regulation of the adeB gene associated with a decrease in susceptibility to non-fluoroquinolone antibiotics. These data illustrate the propensity for A. baumannii to develop multi-drug resistance rapidly.
OBJECTIVES: To investigate the mechanism of ciprofloxacin resistance in isolates of Acinetobacter baumannii during two hospital outbreaks and to determine the expression level of the gene encoding the AdeB efflux pump. METHODS: Isolates were previously typed by PFGE and their MICs determined by broth microdilution. The gyrA and parC genes were sequenced and the adeB gene examined by real-time reverse transcription PCR (RT-PCR). RESULTS: Two clonal lineages were responsible for the two hospital outbreaks. In both outbreaks, ciprofloxacin susceptibility was reduced during the course of the outbreak when compared with the index isolates. Mutations in gyrA and parC were found to have occurred during the outbreak. The MICs of non-fluoroquinolone antibiotics were raised in one clonal lineage and this was associated with a >10-fold increase in mRNA transcripts for adeB. CONCLUSIONS: We have witnessed the appearance of gyrA and parC mutations during outbreaks of A. baumannii. In parallel with these mutations, we observed up-regulation of the adeB gene associated with a decrease in susceptibility to non-fluoroquinolone antibiotics. These data illustrate the propensity for A. baumannii to develop multi-drug resistance rapidly.
Authors: Paul G Higgins; Thamarai Schneiders; Axel Hamprecht; Harald Seifert Journal: Antimicrob Agents Chemother Date: 2010-10-04 Impact factor: 5.191
Authors: Paul G Higgins; Will Stubbings; Hilmar Wisplinghoff; Harald Seifert Journal: Antimicrob Agents Chemother Date: 2010-01-25 Impact factor: 5.191
Authors: Alejandro Beceiro; Enrique Llobet; Jesús Aranda; José Antonio Bengoechea; Michel Doumith; Michael Hornsey; Hiran Dhanji; Henrik Chart; Germán Bou; David M Livermore; Neil Woodford Journal: Antimicrob Agents Chemother Date: 2011-05-16 Impact factor: 5.191
Authors: Kristine M Hujer; Andrea M Hujer; Andrea Endimiani; Jodi M Thomson; Mark D Adams; Karrie Goglin; Philip N Rather; Thuy-Trang D Pennella; Christian Massire; Mark W Eshoo; Rangarajan Sampath; Lawrence B Blyn; David J Ecker; Robert A Bonomo Journal: J Clin Microbiol Date: 2009-03-18 Impact factor: 5.948