Literature DB >> 15354445

Towards a miniaturised system for dynamic field gradient focused separation of proteins.

P Myers1, K D Bartle.   

Abstract

Separation and focusing of proteins is described in a miniaturised dynamic field gradient focusing device with a 2.5 cm x 0.1 cm channel filled with a porous polymer monolith. The separation channel is in contact with a parallel electric field channel with five individually addressable electrodes through a porous glass membrane so that a variable field can be generated that drives charged proteins electroosmotically against a constant hydrodynamic flow. Separated pre-stained proteins were detected by means of a digital camera and background subtraction.

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Year:  2004        PMID： 15354445     DOI： 10.1016/j.chroma.2004.04.069

Source DB:  PubMed          Journal:  J Chromatogr A        ISSN： 0021-9673            Impact factor:   4.759


  8 in total

1.  Characterization of voltage degradation in dynamic field gradient focusing.

Authors:  Jeffrey M Burke; Cornelius F Ivory
Journal:  Electrophoresis       Date:  2008-03       Impact factor: 3.535

2.  Assessing the scalability of dynamic field gradient focusing by linear modeling.

Authors:  Noah I Tracy; Cornelius F Ivory
Journal:  J Sep Sci       Date:  2008-02       Impact factor: 3.645

3.  Design and construction of a preparative-scale dynamic field gradient focusing apparatus.

Authors:  Noah I Tracy; Zheng Huang; Cornelius F Ivory
Journal:  Biotechnol Prog       Date:  2008-01-29

4.  Influence of the semi-permeable membrane on the performance of dynamic field gradient focusing.

Authors:  Jeffrey M Burke; Cornelius F Ivory
Journal:  Electrophoresis       Date:  2010-03       Impact factor: 3.535

5.  Development of a membrane-less dynamic field gradient focusing device for the separation of low-molecular-weight molecules.

Authors:  Jeffrey M Burke; Colin D Smith; Cornelius F Ivory
Journal:  Electrophoresis       Date:  2010-03       Impact factor: 3.535

6.  Protein separation using preparative-scale dynamic field gradient focusing.

Authors:  Noah I Tracy; Cornelius F Ivory
Journal:  Electrophoresis       Date:  2008-07       Impact factor: 3.535

7.  Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice.

Authors:  Stacy M Kenyon; Noah G Weiss; Mark A Hayes
Journal:  Electrophoresis       Date:  2012-04       Impact factor: 3.535

8.  Simultaneous separation of negatively and positively charged species in dynamic field gradient focusing using a dual polarity electric field.

Authors:  Jeffrey M Burke; Zheng Huang; Cornelius F Ivory
Journal:  Anal Chem       Date:  2009-10-01       Impact factor: 6.986
  8 in total

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