The dependence of osteoblastic response on variations in the chemical composition and physical properties of hydroxyapatite.

Two synthetic hydroxyapatite powders (A and B), supplied by different manufacturers, were physically and chemically characterized before being die pressed and sintered at 1250 degrees C. The powders were characterized using X-ray diffraction (XRD), infrared spectroscopy (IRS), X-ray fluorescence, surface area analysis (BET), particle size analysis and scanning electron microscopy (SEM). The materials were then pressed and sintered to produce hydroxyapatite discs of similar densities and grain sizes for in vitro evaluation. The ceramics were seeded with osteoblastic cells and after 15 days in culture the cell morphology was assessed using scanning electron microscopy (SEM), the ultrastructure of the cells was studied using transmission electron microscopy (TEM) with EDAX, and the rate of cell growth was assessed using biochemical techniques. The results clearly showed that the rate of cell proliferation but not the rate of alkaline phosphatase production, was highly dependent on the composition of the hydroxyapatite powders that were used to make the ceramic discs. The ultrastructural studies confirmed the relative viabilities of the cells and the nature of the ceramic interface indicating visually the marked differences in the performance of the two materials.