| Literature DB >> 15345295 |
Abstract
Recent developments in the separation of peptides by high-performance liquid chromatography (HPLC) using polar sorbents with less polar eluents are summarized in this review. This separation mode is now commonly referred to as Hydrophilic-Interaction Chromatography (HILIC). The retention mechanism and chromatographic behavior of polar solutes under HILIC conditions are studied on TSKgel Amide-80 columns, which consist of carbamoyl groups bonded to a silica gel matrix, using a mixture of acetonitrile (MeCN)-water containing 0.1% trifluoroacetic acid (TFA). Some applications are given in peptide field using Hydrophilic-Interaction Chromatography.Entities:
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Year: 2004 PMID： 15345295 DOI： 10.1016/j.jbbm.2004.01.006
Source DB: PubMed Journal: J Biochem Biophys Methods ISSN： 0165-022X