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Literature DB >> 21890140

Protein UTLC-MALDI-MS using thin films of submicrometer silica particles.

Zhaorui Zhang¹, Saliya N Ratnayaka, Mary J Wirth.

Abstract

Slides for ultra thin-layer chromatography (UTLC) were made by coating nonporous silica particles, chemically modified with polyacrylamide, as 15 μm films on glass or silicon. Three proteins, myoglobin, cytochrome c and lysozyme, are nearly baseline resolved by the mechanism of hydrophilic interaction chromatography. A plate height as low as 3 μm, with 3900 plates, is observed in 14 mm. Varying silica particle diameter among 900, 700 and 350 nm showed that decreasing particle diameter slightly improves resolution but slows the separation. Matrix-assisted laser desorption/ionization (MALDI)-MS of the proteins after separation is demonstrated by wicking sufficient sinapinic acid into the separation medium.

Entities: Chemical Disease Gene Species

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Year: 2011 PMID： 21890140 PMCID： PMC3196342 DOI： 10.1016/j.chroma.2011.07.098

Source DB: PubMed Journal: J Chromatogr A ISSN： 0021-9673 Impact factor: 4.759

19 in total

1. Engineered anisotropic microstructures for ultrathin-layer chromatography.

Authors: S R Jim; M T Taschuk; G E Morlock; L W Bezuidenhout; W Schwack; M J Brett
Journal: Anal Chem Date: 2010-06-15 Impact factor: 6.986

2. Electrospun glassy carbon ultra-thin layer chromatography devices.

Authors: Jonathan E Clark; Susan V Olesik
Journal: J Chromatogr A Date: 2010-07-02 Impact factor: 4.759

3. Monolithic porous polymer layer for the separation of peptides and proteins using thin-layer chromatography coupled with MALDI-TOF-MS.

Authors: Rania Bakry; Günther K Bonn; Dieudonne Mair; Frantisek Svec
Journal: Anal Chem Date: 2007-01-15 Impact factor: 6.986

4. Sub-2 microm porous and nonporous particles for fast separation in reversed-phase high performance liquid chromatography.

Authors: Naijun Wu; Yansheng Liu; Milton L Lee
Journal: J Chromatogr A Date: 2006-08-17 Impact factor: 4.759

Review 2. Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques.

Authors: Roman Borisov; Anastasiia Kanateva; Dmitry Zhilyaev
Journal: Front Chem Date: 2021-12-16 Impact factor: 5.221

2 in total

Protein UTLC-MALDI-MS using thin films of submicrometer silica particles.

1. Engineered anisotropic microstructures for ultrathin-layer chromatography.

2. Electrospun glassy carbon ultra-thin layer chromatography devices.

3. Monolithic porous polymer layer for the separation of peptides and proteins using thin-layer chromatography coupled with MALDI-TOF-MS.

4. Sub-2 microm porous and nonporous particles for fast separation in reversed-phase high performance liquid chromatography.

5. Technique for ultrathin layer chromatography using an electrospun, nanofibrous stationary phase.

6. Thin-layer ion-exchange chromatography of proteins.

7. Hydrophilic-interaction chromatography for the separation of peptides, nucleic acids and other polar compounds.

8. [The chromatography of proteins and nucleic acids on paper and thin layers].

9. Protein glycosylation analysis by HILIC-LC-MS of Proteinase K-generated N- and O-glycopeptides.

10. Plate heights below 50 nm for protein electrochromatography using silica colloidal crystals.

1. Polyacrylamide brush layer for hydrophilic interaction liquid chromatography of intact glycoproteins.

Review 2. Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques.