J P Pandey1. 1. Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29425, USA. pandeyj@musc.edu
Abstract
OBJECTIVE: To determine the role of GM and KM genes--genetic markers of immunoglobulin gamma and kappa chains, respectively--in humoral immunity to human cytomegalovirus (HCMV) in patients with systemic sclerosis (SSc; scleroderma). METHODS: A total of 137 Caucasian patients with SSc and 145 ethnically matched controls were genotyped for GM f/3, z/17, n+/23+, n-/23-, KM 1, and KM 3 alleles by polymerase chain reaction-restriction fragment length polymorphism and direct DNA sequencing methods. IgG antibodies to HCMV were measured by an enzyme-linked immunosorbent assay. RESULTS: In SSc patients, GM f,z genotypes were strongly associated with the occurrence of anti-HCMV IgG antibodies. The frequency of the GM f homozygotes was lower (42.2 vs 62.2%; p= 0.02; OR=0.4) and the frequency of the GM f,z heterozygotes was higher (51.1 vs 26.7%; p = 0.006; OR = 2.8) in SSc patients with IgG antibodies to HCMV than in subjects who lacked these antibodies. This association was not observed in the control group. KM and GM n genotypes were not significantly associated with the prevalence of these antibodies. CONCLUSION: GM f,z alleles or alleles in linkage disequilibrium with them influence the generation of IgG antibodies to HCMV in patients with SSc.
OBJECTIVE: To determine the role of GM and KM genes--genetic markers of immunoglobulin gamma and kappa chains, respectively--in humoral immunity to human cytomegalovirus (HCMV) in patients with systemic sclerosis (SSc; scleroderma). METHODS: A total of 137 Caucasian patients with SSc and 145 ethnically matched controls were genotyped for GM f/3, z/17, n+/23+, n-/23-, KM 1, and KM 3 alleles by polymerase chain reaction-restriction fragment length polymorphism and direct DNA sequencing methods. IgG antibodies to HCMV were measured by an enzyme-linked immunosorbent assay. RESULTS: In SSc patients, GM f,z genotypes were strongly associated with the occurrence of anti-HCMV IgG antibodies. The frequency of the GM f homozygotes was lower (42.2 vs 62.2%; p= 0.02; OR=0.4) and the frequency of the GM f,z heterozygotes was higher (51.1 vs 26.7%; p = 0.006; OR = 2.8) in SSc patients with IgG antibodies to HCMV than in subjects who lacked these antibodies. This association was not observed in the control group. KM and GM n genotypes were not significantly associated with the prevalence of these antibodies. CONCLUSION:GM f,z alleles or alleles in linkage disequilibrium with them influence the generation of IgG antibodies to HCMV in patients with SSc.
Authors: Paolo Muratori; Susan E Sutherland; Luigi Muratori; Alessandro Granito; Marcello Guidi; Georges Pappas; Marco Lenzi; Francesco B Bianchi; Janardan P Pandey Journal: J Virol Date: 2006-05 Impact factor: 5.103
Authors: Janardan P Pandey; Yuqun Luo; Robert C Elston; Yuping Wu; Frances Hite Philp; Jacquie Astemborski; David L Thomas; Dale M Netski Journal: Hum Immunol Date: 2008-03-10 Impact factor: 2.850
Authors: Geertje M Bartelds; Els de Groot; Michael T Nurmohamed; Margreet H L Hart; Peter H van Eede; Carla A Wijbrandts; Jakob B A Crusius; Ben A C Dijkmans; Paul Peter Tak; Lucien Aarden; Gerrit J Wolbink Journal: Arthritis Res Ther Date: 2010-12-27 Impact factor: 5.156
Authors: Janardan P Pandey; Navtej Kaur; Sandra Costa; Julia Amorim; Rui Nabico; Paulo Linhares; Rui Vaz; Marta Viana-Pereira; Rui M Reis Journal: Oncoimmunology Date: 2014-05-23 Impact factor: 8.110