Literature DB >> 15338282

An improved method for detection of Leishmania amastigotes by an antibody probe against the small subunit of leishmanial ribonucleotide reductase.

Shu-Ching Chang1, Oleg Kuzmenok, Su-Chi Chiang, Sho Tone Lee.   

Abstract

By taking advantage of an antibody raised against the small M2 subunit of ribonucleotide reductase of Leishmania that reacts with the enzyme in the nucleus of the parasite but does not cross-react with the same enzyme of the host macrophage, an improved fluorescence-staining method is developed for enumeration of leishmanial amastigotes inside the macrophage. The method offers an accurate and easy way of counting, compared with Giemsa staining.

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Year:  2004        PMID： 15338282     DOI： 10.1007/s00436-004-1184-7

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  17 in total

1.  Cloning and functional analysis of the ribonucleotide reductase gene small subunit from hydroxyurea-resistant Leishmania mexicana amazonensis.

Authors:  L F Lye; Y H Hsieh; K E Su; S T Lee
Journal:  Mol Biochem Parasitol       Date:  1997-12-01       Impact factor: 1.759

2.  Multiplication of a human parasite (Leishmania donovani) in phagolysosomes of hamster macrophages in vitro.

Authors:  K P Chang; D M Dwyer
Journal:  Science       Date:  1976-08-20       Impact factor: 47.728

3.  Accurate quantitation of Leishmania infection in cultured cells by flow cytometry.

Authors:  F Guinet; A Louise; H Jouin; J C Antoine; C W Roth
Journal:  Cytometry       Date:  2000-03-01

4.  The role of IL-10 in promoting disease progression in leishmaniasis.

Authors:  M M Kane; D M Mosser
Journal:  J Immunol       Date:  2001-01-15       Impact factor: 5.422

5.  Murine macrophages produce secretory leukocyte protease inhibitor during clearance of apoptotic cells: implications for resolution of the inflammatory response.

Authors:  Chikako Odaka; Toshiaki Mizuochi; Jingxuan Yang; Aihao Ding
Journal:  J Immunol       Date:  2003-08-01       Impact factor: 5.422

6.  Characterization of Trichomonas vaginalis virus proteins in the pathogenic protozoan T. vaginalis.

Authors:  H W Liu; Y D Chu; J H Tai
Journal:  Arch Virol       Date:  1998       Impact factor: 2.574

7.  Enhanced replication of Leishmania amazonensis amastigotes in gamma interferon-stimulated murine macrophages: implications for the pathogenesis of cutaneous leishmaniasis.

Authors:  Hai Qi; Jiaxiang Ji; Nanchaya Wanasen; Lynn Soong
Journal:  Infect Immun       Date:  2004-02       Impact factor: 3.441

8.  Identification of Leishmania species by a specific DNA probe that is conserved only in the maxicircle DNA of human-infective Leishmania parasites.

Authors:  S T Lee; S C Chiang; A K Singh; H Y Liu
Journal:  J Infect Dis       Date:  1995-09       Impact factor: 5.226

9.  Lipophosphoglycan from Leishmania mexicana promastigotes binds to members of the CR3, p150,95 and LFA-1 family of leukocyte integrins.

Authors:  P Talamás-Rohana; S D Wright; M R Lennartz; D G Russell
Journal:  J Immunol       Date:  1990-06-15       Impact factor: 5.422

10.  Immunocytochemical evidence for the cytoplasmic localization and differential expression during the cell cycle of the M1 and M2 subunits of mammalian ribonucleotide reductase.

Authors:  Y Engström; B Rozell
Journal:  EMBO J       Date:  1988-06       Impact factor: 11.598
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