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Literature DB >> 15329383

Genomic representations using concatenates of Type IIB restriction endonuclease digestion fragments.

Torstein Tengs¹, Thomas LaFramboise, Robert B Den, David N Hayes, Jianhua Zhang, Saikat DebRoy, Robert C Gentleman, Keith O'Neill, Bruce Birren, Matthew Meyerson.

Abstract

We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by concatenation of restriction digests, or RECORD, is an approach to sample the fragments generated by cleavage with these enzymes. Here, we show that the RECORD libraries may be used for digital karyotyping and for pathogen identification by computational subtraction.

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Year: 2004 PMID： 15329383 PMCID： PMC516078 DOI： 10.1093/nar/gnh120

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

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