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Literature DB >> 15317990

Segmental isotopic labeling for structural biological applications of NMR.

David Cowburn¹, Alexander Shekhtman, Rong Xu, Jennifer J Ottesen, Tom W Muir.

Abstract

This chapter describes the preparation of precursor domains for the formation of multidomain segmentally labeled proteins by protein ligation.

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Year: 2004 PMID： 15317990 DOI： 10.1385/1-59259-809-9:047

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

12 in total

1. Efficient segmental isotope labeling of multi-domain proteins using Sortase A.

Authors: Lee Freiburger; Miriam Sonntag; Janosch Hennig; Jian Li; Peijian Zou; Michael Sattler
Journal: J Biomol NMR Date: 2015-08-30 Impact factor: 2.835

Review 2. Solution NMR of large molecules and assemblies.

Authors: Mark P Foster; Craig A McElroy; Carlos D Amero
Journal: Biochemistry Date: 2007-01-16 Impact factor: 3.162

3. Total chemical synthesis and biophysical characterization of the minimal isoform of the KChIP2 potassium channel regulatory subunit.

Authors: Sudarshan Rajagopal; Stephen B H Kent
Journal: Protein Sci Date: 2007-07-27 Impact factor: 6.725

9. An efficient on-column expressed protein ligation strategy: application to segmental triple labeling of human apolipoprotein E3.

Authors: Wentao Zhao; Yonghong Zhang; Chunxian Cui; Qianqian Li; Jianjun Wang
Journal: Protein Sci Date: 2008-02-27 Impact factor: 6.725

10. Utilizing a Baculovirus/Insect Cell Expression System and Expressed Protein Ligation (EPL) for Protein Semisynthesis.

Authors: Marie Butts; Nam Chu
Journal: Curr Protoc Date: 2022-01

Segmental isotopic labeling for structural biological applications of NMR.

1. Efficient segmental isotope labeling of multi-domain proteins using Sortase A.

Review 2. Solution NMR of large molecules and assemblies.

3. Total chemical synthesis and biophysical characterization of the minimal isoform of the KChIP2 potassium channel regulatory subunit.

4. N-terminal cysteinyl proteins can be prepared using thrombin cleavage.

5. Asymmetric methyl group labeling as a probe of membrane protein homo-oligomers by NMR spectroscopy.

6. Chemical ligation of the influenza M2 protein for solid-state NMR characterization of the cytoplasmic domain.

7. The C-terminal helices of heat shock protein 70 are essential for J-domain binding and ATPase activation.

8. Change in the Molecular Dimension of a RAGE-Ligand Complex Triggers RAGE Signaling.

9. An efficient on-column expressed protein ligation strategy: application to segmental triple labeling of human apolipoprotein E3.

10. Utilizing a Baculovirus/Insect Cell Expression System and Expressed Protein Ligation (EPL) for Protein Semisynthesis.