Literature DB >> 1531384

A new method for the isolation of recombinant baculovirus.

G Patel1, K Nasmyth, N Jones.   

Abstract

An improved method for the isolation of baculovirus recombinants is described. The method involves the replication and maintenance of the baculovirus genome in the yeast Saccharomyces cerevisiae which was accomplished by the isolation of a baculovirus recombinant containing yeast ARS and CEN sequences ensuring stable replication in yeast and a URA3 selectable marker. The viral DNA maintained its ability to replicate in insect cells. An efficient and rapid selection system was set up, to isolate viral recombinants in yeast; DNA from selected yeast colonies was transfected into insect cells to obtain recombinant virus. We demonstrate the utility of this system by isolating recombinant viruses that express two different members of the CREB/ATF family of transcription factors.

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Year:  1992        PMID： 1531384      PMCID： PMC310331          DOI： 10.1093/nar/20.1.97

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  39 in total

1.  Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector.

Authors:  D Zuidema; A Schouten; M Usmany; A J Maule; G J Belsham; J Roosien; E C Klinge-Roode; J W van Lent; J M Vlak
Journal:  J Gen Virol       Date:  1990-10       Impact factor: 3.891

2.  Getting started with yeast.

Authors:  F Sherman
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

3.  High-efficiency transformation of yeast by electroporation.

Authors:  D M Becker; L Guarente
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

4.  Activation in vitro of RNA polymerase II and III directed transcription by baculovirus produced E1A protein.

Authors:  G Patel; N C Jones
Journal:  Nucleic Acids Res       Date:  1990-05-25       Impact factor: 16.971

5.  A specific member of the ATF transcription factor family can mediate transcription activation by the adenovirus E1a protein.

Authors:  F Liu; M R Green
Journal:  Cell       Date:  1990-06-29       Impact factor: 41.582

6.  Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors.

Authors:  P A Kitts; M D Ayres; R D Possee
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

7.  Heterodimer formation between CREB and JUN proteins.

Authors:  D M Benbrook; N C Jones
Journal:  Oncogene       Date:  1990-03       Impact factor: 9.867

8.  The cellular transcription factor CREB corresponds to activating transcription factor 47 (ATF-47) and forms complexes with a group of polypeptides related to ATF-43.

Authors:  H C Hurst; N Masson; N C Jones; K A Lee
Journal:  Mol Cell Biol       Date:  1990-12       Impact factor: 4.272

9.  Multiple cDNA clones encoding nuclear proteins that bind to the tax-dependent enhancer of HTLV-1: all contain a leucine zipper structure and basic amino acid domain.

Authors:  T Yoshimura; J Fujisawa; M Yoshida
Journal:  EMBO J       Date:  1990-08       Impact factor: 11.598

10.  Cloning and in vivo expression of the human GART gene using yeast artificial chromosomes.

Authors:  A Gnirke; T S Barnes; D Patterson; D Schild; T Featherstone; M V Olson
Journal:  EMBO J       Date:  1991-07       Impact factor: 11.598
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  14 in total

1.  Improving baculovirus recombination.

Authors:  Yuguang Zhao; David A G Chapman; Ian M Jones
Journal:  Nucleic Acids Res       Date:  2003-01-15       Impact factor: 16.971

2.  Construction of baculovirus recombinants.

Authors:  P A Kitts
Journal:  Cytotechnology       Date:  1996-01       Impact factor: 2.058

3.  A bi-cistronic baculovirus expression vector for improved recombinant protein production.

Authors:  Tzong-Yuan Wu; Ying-Ju Chen; Chao-Yi Teng; Wen-Shuo Chen; Oliver Villaflores
Journal:  Bioeng Bugs       Date:  2012-03-01

Review 4.  Manipulation of baculovirus vectors.

Authors:  C L Merrington; M J Bailey; R D Possee
Journal:  Mol Biotechnol       Date:  1997-12       Impact factor: 2.695

5.  Direct cloning into the Autographa californica nuclear polyhedrosis virus for generation of recombinant baculoviruses.

Authors:  W J Ernst; R M Grabherr; H W Katinger
Journal:  Nucleic Acids Res       Date:  1994-07-25       Impact factor: 16.971

6.  p300 and ATF-2 are components of the DRF complex, which regulates retinoic acid- and E1A-mediated transcription of the c-jun gene in F9 cells.

Authors:  H Kawasaki; J Song; R Eckner; H Ugai; R Chiu; K Taira; Y Shi; N Jones; K K Yokoyama
Journal:  Genes Dev       Date:  1998-01-15       Impact factor: 11.361

7.  PAK1, a gene that can regulate p53 activity in yeast.

Authors:  S Thiagalingam; K W Kinzler; B Vogelstein
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-20       Impact factor: 11.205

8.  The 35-kilodalton protein gene (p35) of Autographa californica nuclear polyhedrosis virus and the neomycin resistance gene provide dominant selection of recombinant baculoviruses.

Authors:  R A Lerch; P D Friesen
Journal:  Nucleic Acids Res       Date:  1993-04-25       Impact factor: 16.971

9.  Improved generation of recombinant baculovirus genomes in Escherichia coli.

Authors:  Kari J Airenne; Erik Peltomaa; Vesa P Hytönen; Olli H Laitinen; Seppo Ylä-Herttuala
Journal:  Nucleic Acids Res       Date:  2003-09-01       Impact factor: 16.971

10.  Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli.

Authors:  V A Luckow; S C Lee; G F Barry; P O Olins
Journal:  J Virol       Date:  1993-08       Impact factor: 5.103
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