Literature DB >> 12930975

Improved generation of recombinant baculovirus genomes in Escherichia coli.

Kari J Airenne1, Erik Peltomaa, Vesa P Hytönen, Olli H Laitinen, Seppo Ylä-Herttuala.   

Abstract

An improved method for the generation of recombinant baculoviruses by Tn7-mediated transposition is described. The method is based on the modified donor vector (pBVboost) and an improved selection scheme of the baculovirus bacmids in Escherichia coli with a mutated SacB gene. Recombinant bacmids can be generated at a frequency of approximately 10(7)/microg of donor vector with a negligible background. This easy-to-use and efficient pBVboost system provides the basis for a high-throughput generation of recombinant baculoviruses as well as a more convenient way to produce single viruses. The introduced selection scheme is also useful for the construction of other vectors by transposition in E.coli.

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Year:  2003        PMID： 12930975      PMCID： PMC212821          DOI： 10.1093/nar/gng102

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

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Authors:  T C Peakman; R A Harris; D R Gewert
Journal:  Nucleic Acids Res       Date:  1992-02-11       Impact factor: 16.971

2.  The admid system: generation of recombinant adenoviruses by Tn7-mediated transposition in E. coli.

Authors:  C A Richards; C E Brown; J P Cogswell; M P Weiner
Journal:  Biotechniques       Date:  2000-07       Impact factor: 1.993

Review 3.  Current methods for manipulating baculoviruses.

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Journal:  Biotechnology (N Y)       Date:  1994-01

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Authors:  W J Ernst; R M Grabherr; H W Katinger
Journal:  Nucleic Acids Res       Date:  1994-07-25       Impact factor: 16.971

5.  Efficient generation of infectious recombinant baculoviruses by site-specific transposon-mediated insertion of foreign genes into a baculovirus genome propagated in Escherichia coli.

Authors:  V A Luckow; S C Lee; G F Barry; P O Olins
Journal:  J Virol       Date:  1993-08       Impact factor: 5.103

6.  A new method for the isolation of recombinant baculovirus.

Authors:  G Patel; K Nasmyth; N Jones
Journal:  Nucleic Acids Res       Date:  1992-01-11       Impact factor: 16.971

7.  Production of recombinant avidin in Escherichia coli.

Authors:  K J Airenne; P Sarkkinen; E L Punnonen; M S Kulomaa
Journal:  Gene       Date:  1994-06-24       Impact factor: 3.688

8.  Production of human beta interferon in insect cells infected with a baculovirus expression vector.

Authors:  G E Smith; M D Summers; M J Fraser
Journal:  Mol Cell Biol       Date:  1983-12       Impact factor: 4.272

9.  A method for producing recombinant baculovirus expression vectors at high frequency.

Authors:  P A Kitts; R D Possee
Journal:  Biotechniques       Date:  1993-05       Impact factor: 1.993

10.  Baculovirus and insect cell gene expression: review of baculovirus biotechnology.

Authors:  R M Patterson; J K Selkirk; B A Merrick
Journal:  Environ Health Perspect       Date:  1995 Jul-Aug       Impact factor: 9.031
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  20 in total

1.  Baculoviral capsid display of His-tagged ZnO inorganic binding peptide.

Authors:  Lei Song; Yingying Liu; Jinchun Chen
Journal:  Cytotechnology       Date:  2010-04-21       Impact factor: 2.058

2.  A bi-cistronic baculovirus expression vector for improved recombinant protein production.

Authors:  Tzong-Yuan Wu; Ying-Ju Chen; Chao-Yi Teng; Wen-Shuo Chen; Oliver Villaflores
Journal:  Bioeng Bugs       Date:  2012-03-01

Review 3.  Baculovirus: an insect-derived vector for diverse gene transfer applications.

Authors:  Kari J Airenne; Yu-Chen Hu; Thomas A Kost; Richard H Smith; Robert M Kotin; Chikako Ono; Yoshiharu Matsuura; Shu Wang; Seppo Ylä-Herttuala
Journal:  Mol Ther       Date:  2013-02-26       Impact factor: 11.454

4.  Enhanced expression of recombinant proteins utilizing a modified baculovirus expression vector.

Authors:  Prabhakar Tiwari; Shalini Saini; Sanatan Upmanyu; Biju Benjamin; Ruchi Tandon; Kulvinder Singh Saini; Sudhir Sahdev
Journal:  Mol Biotechnol       Date:  2010-09       Impact factor: 2.695

5.  Engineering the transposition-based baculovirus expression vector system for higher efficiency protein production from insect cells.

Authors:  Jennifer L Mehalko; Dominic Esposito
Journal:  J Biotechnol       Date:  2016-09-09       Impact factor: 3.307

6.  Novel vascular endothelial growth factor D variants with increased biological activity.

Authors:  Pyry I Toivanen; Tiina Nieminen; Lenita Viitanen; Annamari Alitalo; Miia Roschier; Suvi Jauhiainen; Johanna E Markkanen; Olli H Laitinen; Tomi T Airenne; Tiina A Salminen; Mark S Johnson; Kari J Airenne; Seppo Ylä-Herttuala
Journal:  J Biol Chem       Date:  2009-04-14       Impact factor: 5.157

Review 7.  Baculovirus as versatile vectors for protein expression in insect and mammalian cells.

Authors:  Thomas A Kost; J Patrick Condreay; Donald L Jarvis
Journal:  Nat Biotechnol       Date:  2005-05       Impact factor: 54.908

8.  rDNA-directed integration by an HIV-1 integrase--I-PpoI fusion protein.

Authors:  Diana Schenkwein; Vesa Turkki; Mervi K Ahlroth; Oskari Timonen; Kari J Airenne; Seppo Ylä-Herttuala
Journal:  Nucleic Acids Res       Date:  2012-12-28       Impact factor: 16.971

9.  Experimental Evaluation of an Interferometric Light Microscopy Particle Counter for Titering and Characterization of Virus Preparations.

Authors:  Vesa Turkki; Elisa Alppila; Seppo Ylä-Herttuala; Hanna P Lesch
Journal:  Viruses       Date:  2021-05-19       Impact factor: 5.048

10.  Use of bacterial artificial chromosomes in baculovirus research and recombinant protein expression: current trends and future perspectives.

Authors:  Polly Roy; Rob Noad
Journal:  ISRN Microbiol       Date:  2012-09-12
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