OBJECTIVE: Two main subpopulations of human blood monocytes are distinguished on the basis of CD14 and CD16 expression: the major population with enhanced expression of CD14 (CD14++ monocytes) and the minor one with a weak expression of CD14 coexpressing CD16 (CD14+/CD16+ monocytes). As monocytes and macrophages are involved in antitumor response of the host, we assessed the ability of CD14+/CD16+ monocytes to produce cytokines (intracellular expression, release) and reactive oxygen and nitrogen (ROI, RNI) intermediates following stimulation in vitro with tumor cells. MATERIALS AND METHODS: Monocytes were isolated by elutriation and their subpopulations by FACS sorting. Monocytes and their subpopulations were cocultured with tumor cells. Cytokine (TNF-alpha, IL-12, and IL-10) production was assessed by determination of intracellular protein expression by flow cytometry, and release by ELISA. ROI induction was detected by chemiluminescence and O2- production by flow cytometry, whereas RNI by intracellular expression of inducible NO synthase (iNOS) and nitric oxide (NO) release assessed colorimetrically. RESULTS: CD14+/CD16+ monocytes stimulated with tumor cells showed significantly enhanced production of TNF-alpha, IL-12p40, IL-12p70 (intracellular expression, release), whereas little IL-10 release was observed. CD14+/CD16+ subpopulation did not produce ROI, but showed an increased iNOS expression and NO release. CD14+/CD16+ monocytes also exhibited enhanced cytotoxic and cytostatic activities against tumor cells. CONCLUSIONS: CD14+/CD16+ cells constitute the main subpopulation of blood monocytes involved in antitumor response as judged by enhanced production of proinflammatory cytokines, RNI, and increased cytotoxic/cytostatic activity.
OBJECTIVE: Two main subpopulations of human blood monocytes are distinguished on the basis of CD14 and CD16 expression: the major population with enhanced expression of CD14 (CD14++ monocytes) and the minor one with a weak expression of CD14 coexpressing CD16 (CD14+/CD16+ monocytes). As monocytes and macrophages are involved in antitumor response of the host, we assessed the ability of CD14+/CD16+ monocytes to produce cytokines (intracellular expression, release) and reactive oxygen and nitrogen (ROI, RNI) intermediates following stimulation in vitro with tumor cells. MATERIALS AND METHODS: Monocytes were isolated by elutriation and their subpopulations by FACS sorting. Monocytes and their subpopulations were cocultured with tumor cells. Cytokine (TNF-alpha, IL-12, and IL-10) production was assessed by determination of intracellular protein expression by flow cytometry, and release by ELISA. ROI induction was detected by chemiluminescence and O2- production by flow cytometry, whereas RNI by intracellular expression of inducible NO synthase (iNOS) and nitric oxide (NO) release assessed colorimetrically. RESULTS:CD14+/CD16+ monocytes stimulated with tumor cells showed significantly enhanced production of TNF-alpha, IL-12p40, IL-12p70 (intracellular expression, release), whereas little IL-10 release was observed. CD14+/CD16+ subpopulation did not produce ROI, but showed an increased iNOS expression and NO release. CD14+/CD16+ monocytes also exhibited enhanced cytotoxic and cytostatic activities against tumor cells. CONCLUSIONS:CD14+/CD16+ cells constitute the main subpopulation of blood monocytes involved in antitumor response as judged by enhanced production of proinflammatory cytokines, RNI, and increased cytotoxic/cytostatic activity.
Authors: Graham Thomas; Robert Tacke; Catherine C Hedrick; Richard N Hanna Journal: Arterioscler Thromb Vasc Biol Date: 2015-04-02 Impact factor: 8.311
Authors: Claire Lastrucci; Alan Bénard; Luciana Balboa; Karine Pingris; Shanti Souriant; Renaud Poincloux; Talal Al Saati; Voahangy Rasolofo; Pablo González-Montaner; Sandra Inwentarz; Eduardo Jose Moraña; Ivanela Kondova; Frank A W Verreck; Maria del Carmen Sasiain; Olivier Neyrolles; Isabelle Maridonneau-Parini; Geanncarlo Lugo-Villarino; Céline Cougoule Journal: Cell Res Date: 2015-10-20 Impact factor: 25.617
Authors: Moritz Wildgruber; Hakho Lee; Aleksey Chudnovskiy; Tae-Jong Yoon; Martin Etzrodt; Mikael J Pittet; Matthias Nahrendorf; Kevin Croce; Peter Libby; Ralph Weissleder; Filip K Swirski Journal: PLoS One Date: 2009-05-22 Impact factor: 3.240