| Literature DB >> 15297874 |
Róisín M Owens1, Gareth Pritchard, Paul Skipp, Michelle Hodey, Sean R Connell, Knud H Nierhaus, C David O'Connor.
Abstract
BipA is a highly conserved protein with global regulatory properties in Escherichia coli. We show here that it functions as a translation factor that is required specifically for the expression of the transcriptional modulator Fis. BipA binds to ribosomes at a site that coincides with that of elongation factor G and has a GTPase activity that is sensitive to high GDP:GTP ratios and stimulated by 70S ribosomes programmed with mRNA and aminoacylated tRNAs. The growth rate-dependent induction of BipA allows the efficient expression of Fis, thereby modulating a range of downstream processes, including DNA metabolism and type III secretion. We propose a model in which BipA destabilizes unusually strong interactions between the 5' untranslated region of fis mRNA and the ribosome. Since BipA spans phylogenetic domains, transcript-selective translational control for the 'fast-track' expression of specific mRNAs may have wider significance.Entities:
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Year: 2004 PMID： 15297874 PMCID： PMC514516 DOI： 10.1038/sj.emboj.7600343
Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598