Literature DB >> 15297485

Selected pool of peptides from ESAT-6 and CFP-10 proteins for detection of Mycobacterium tuberculosis infection.

Paolo Scarpellini1, Silvana Tasca, Laura Galli, Alberto Beretta, Adriano Lazzarin, Claudio Fortis.   

Abstract

We have validated a new test for detecting Mycobacterium tuberculosis infection. A pool of synthetic peptides derived from ESAT-6 and CFP-10 proteins was used to detect the number of specific gamma interferon-producing T cells by means of an enzyme-linked immunospot assay. Sixty-eight individuals positive for M. tuberculosis infection, either human immunodeficiency virus-seropositive or -seronegative, were studied. The test results were highly specific (87.5%) and sensitive (93.1%), more so than a classical lymphoproliferative assay (specificity and sensitivity of 77.27%), opening new possibilities for diagnosis and screening of tuberculosis. Moreover, the test allowed us to distinguish individuals infected with M. tuberculosis from those vaccinated with BCG.

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Year:  2004        PMID： 15297485      PMCID： PMC497651          DOI： 10.1128/JCM.42.8.3469-3474.2004

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  40 in total

1.  Diagnosis of tuberculosis based on the two specific antigens ESAT-6 and CFP10.

Authors:  L A van Pinxteren; P Ravn; E M Agger; J Pollock; P Andersen
Journal:  Clin Diagn Lab Immunol       Date:  2000-03

Review 2.  Immunology of tuberculosis.

Authors:  J L Flynn; J Chan
Journal:  Annu Rev Immunol       Date:  2001       Impact factor: 28.527

3.  Enhanced contact tracing and spatial tracking of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells.

Authors:  A Lalvani; A A Pathan; H Durkan; K A Wilkinson; A Whelan; J J Deeks; W H Reece; M Latif; G Pasvol; A V Hill
Journal:  Lancet       Date:  2001-06-23       Impact factor: 79.321

4.  Multiple epitopes from the Mycobacterium tuberculosis ESAT-6 antigen are recognized by antigen-specific human T cell lines.

Authors:  A S Mustafa; F Oftung; H A Amoudy; N M Madi; A T Abal; F Shaban; I Rosen Krands; P Andersen
Journal:  Clin Infect Dis       Date:  2000-06       Impact factor: 9.079

5.  Tuberculin skin testing compared with T-cell responses to Mycobacterium tuberculosis-specific and nonspecific antigens for detection of latent infection in persons with recent tuberculosis contact.

Authors:  S M Arend; A C Engelhard; G Groot; K de Boer; P Andersen; T H Ottenhoff; J T van Dissel
Journal:  Clin Diagn Lab Immunol       Date:  2001-11

6.  Enumeration of T cells specific for RD1-encoded antigens suggests a high prevalence of latent Mycobacterium tuberculosis infection in healthy urban Indians.

Authors:  A Lalvani; P Nagvenkar; Z Udwadia; A A Pathan; K A Wilkinson; J S Shastri; K Ewer; A V Hill; A Mehta; C Rodrigues
Journal:  J Infect Dis       Date:  2000-12-21       Impact factor: 5.226

Review 7.  Specific immune-based diagnosis of tuberculosis.

Authors:  P Andersen; M E Munk; J M Pollock; T M Doherty
Journal:  Lancet       Date:  2000-09-23       Impact factor: 79.321

8.  Direct ex vivo analysis of antigen-specific IFN-gamma-secreting CD4 T cells in Mycobacterium tuberculosis-infected individuals: associations with clinical disease state and effect of treatment.

Authors:  A A Pathan; K A Wilkinson; P Klenerman; H McShane; R N Davidson; G Pasvol; A V Hill; A Lalvani
Journal:  J Immunol       Date:  2001-11-01       Impact factor: 5.422

9.  Performance of whole blood IFN-gamma test for tuberculosis diagnosis based on PPD or the specific antigens ESAT-6 and CFP-10.

Authors:  I Brock; M E Munk; A Kok-Jensen; P Andersen
Journal:  Int J Tuberc Lung Dis       Date:  2001-05       Impact factor: 2.373

10.  Rapid detection of Mycobacterium tuberculosis infection by enumeration of antigen-specific T cells.

Authors:  A Lalvani; A A Pathan; H McShane; R J Wilkinson; M Latif; C P Conlon; G Pasvol; A V Hill
Journal:  Am J Respir Crit Care Med       Date:  2001-03       Impact factor: 21.405
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  15 in total

1.  ELISPOT-IFN-gamma assay instead of tuberculin skin test for detecting latent Mycobacterium tuberculosis infection in rheumatic patients candidate to anti-TNF-alpha treatment.

Authors:  Stefania Girlanda; Paola Mantegani; Elena Baldissera; Patrizia Aiello; Manuela Ratti; Maria Grazia Sabbadini; Claudio Fortis
Journal:  Clin Rheumatol       Date:  2010-07-20       Impact factor: 2.980

2.  An in-house RD1-based enzyme-linked immunospot-gamma interferon assay instead of the tuberculin skin test for diagnosis of latent Mycobacterium tuberculosis infection.

Authors:  Luigi Codecasa; Paola Mantegani; Laura Galli; Adriano Lazzarin; Paolo Scarpellini; Claudio Fortis
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

3.  Clinical evaluation of a homemade enzyme-linked immunospot assay for the diagnosis of active tuberculosis in China.

Authors:  Xueqiong Wu; Qiaoke Li; Yan Liang; Yourong Yang; Junxian Zhang; Jianqin Liang; Lan Li; Fakuan Tang; Ansheng Wang
Journal:  Mol Biotechnol       Date:  2011-01       Impact factor: 2.695

4.  Specific and randomly derived immunoactive peptide mimotopes of mycobacterial antigens.

Authors:  Archna Sharma; Abhik Saha; Surajit Bhattacharjee; Subrata Majumdar; Sujoy K Das Gupta
Journal:  Clin Vaccine Immunol       Date:  2006-08-17

5.  Comparison of an in-house and a commercial RD1-based ELISPOT-IFN-gamma assay for the diagnosis of Mycobacterium tuberculosis infection.

Authors:  Paola Mantegani; Federica Piana; Luigi Codecasa; Laura Galli; Paolo Scarpellini; Adriano Lazzarin; Daniela Cirillo; Claudio Fortis
Journal:  Clin Med Res       Date:  2006-12

6.  Identification of Mycobacterium tuberculosis PPE68-specific HLA-A*0201-restricted epitopes for tuberculosis diagnosis.

Authors:  Zhi-Liang Duan; Qiang Li; Sina Wang; Xin-Yu Chen; Hui-Fang Liu; Bo-Kun Chen; De-Zhou Li; Xi Huang; Jin-Sheng Wen
Journal:  Curr Microbiol       Date:  2015-02-15       Impact factor: 2.188

7.  Detection of polyfunctional Mycobacterium tuberculosis-specific T cells and association with viral load in HIV-1-infected persons.

Authors:  Cheryl L Day; Nompumelelo Mkhwanazi; Sharon Reddy; Zenele Mncube; Mary van der Stok; Paul Klenerman; Bruce D Walker
Journal:  J Infect Dis       Date:  2008-04-01       Impact factor: 5.226

8.  Use of enzyme-linked immunospot assay with Mycobacterium tuberculosis-specific peptides for diagnosis of recent infection with M. tuberculosis after accidental laboratory exposure.

Authors:  Eliane M S Leyten; Bert Mulder; Corine Prins; Karin Weldingh; Peter Andersen; Tom H M Ottenhoff; Jaap T van Dissel; Sandra M Arend
Journal:  J Clin Microbiol       Date:  2006-03       Impact factor: 5.948

9.  Response to region of difference 1 (RD1) epitopes in human immunodeficiency virus (HIV)-infected individuals enrolled with suspected active tuberculosis: a pilot study.

Authors:  D Vincenti; S Carrara; O Butera; F Bizzoni; R Casetti; E Girardi; D Goletti
Journal:  Clin Exp Immunol       Date:  2007-08-03       Impact factor: 4.330

10.  Enhancement of human antigen-specific memory T-cell responses by interleukin-7 may improve accuracy in diagnosing tuberculosis.

Authors:  Marsha Feske; Rodolfo J Nudelman; Miguel Medina; Justin Lew; Manisha Singh; Jacob Couturier; Edward A Graviss; Dorothy E Lewis
Journal:  Clin Vaccine Immunol       Date:  2008-08-27
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